Anti-CPNE1抗体
产品名称: Anti-CPNE1抗体
英文名称: CPNE1
产品编号: YB--14024R
产品价格: null
产品产地: 中国/美国
品牌商标: Ybscience
更新时间: 2023-08-17T10:29:50
使用范围: 科研使用
上海钰博生物科技有限公司
- 联系人 : 陈环环
- 地址 : 上海市沪闵路6088号龙之梦大厦8楼806室
- 邮编 : 200612
- 所在区域 : 上海
- 电话 : 183****2235
- 传真 : 021-60514606
- 邮箱 : shybio@126.com
Anti-CPNE1抗体
产品编号 | YB-14024R |
英文名称 | CPNE1 |
中文名称 | 钙依赖膜结合蛋白Copine蛋白1抗体 |
别 名 | Copine 1; Copine I; Copine-1; Copine1; CopineI; COPN 1; COPN1; CPN 1; CPN1; CPNE 1; CPNE1; CPNE1_HUMAN; MGC1142. |
规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
说 明 书 | 100ul 200ul |
研究领域 | 肿瘤 细胞生物 信号转导 转运蛋白 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human, Mouse, Rat, Pig, Cow, Sheep, |
产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 59kDa |
细胞定位 | 细胞膜 |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human CPNE1:1-120/537 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
PubMed | PubMed |
产品介绍 | background: Copine I is a member of the copine family of evolutionarily conserved, soluble, calcium-dependent, membrane-binding proteins. Members of the copine family are involved in signal transduction and membrane trafficking. Copine I is ubiquitously expressed and contains two N-terminal C2 domains and one C-terminal VWFA (von Willebrand factor A) domain, which is also referred to as the A domain or the core domain. As is characteristic of the copine family, copine I functions in membrane trafficking and is capable of binding phospholipids in a calcium-dependent manner. Copine I is also able to bind liposomes. Via its VWFA domain, copine I directly interacts with the ubiquitin-conjugating enzyme, UBC12, and may play a role in the regulation of TNF alpha-signaling. Function: May function in membrane trafficking. Exhibits calcium-dependent phospholipid binding properties. Subunit: Interacts with CCDC22. Similarity: Belongs to the copine family. Contains 2 C2 domains. Contains 1 VWFA domain. SWISS: Q99829 Gene ID: 615677 Database links: Entrez Gene: 615677 Cow Entrez Gene: 477213 Dog Entrez Gene: 8904 Human Entrez Gene: 266692 Mouse Entrez Gene: 100233174 Pig Entrez Gene: 100328904 Rabbit Omim: 604205 Human SwissProt: Q99829 Human SwissProt: Q8C166 Mouse Unigene: 246413 Human Unigene: 700188 Human Unigene: 27660 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品图片 |
Protein: spleen(mouse) lysate at 40ug;
Primary: rabbit Anti-CPNE1 (bs-14024R) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size: 59 kD Observed band size: 59 kD Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CPNE1 Polyclonal Antibody, Unconjugated(bs-14024R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CPNE1 Polyclonal Antibody, Unconjugated(bs-14024R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CPNE1 Polyclonal Antibody, Unconjugated(bs-14024R) 1:400, overnight at 4°C, followed by conjugation to the secondary |