Anti-Dopamine Receptor D1抗体
产品名称: Anti-Dopamine Receptor D1抗体
英文名称: Dopamine Receptor D1
产品编号: YB-10610R
产品价格: null
产品产地: 中国/美国
品牌商标: Ybscience
更新时间: 2023-08-17T10:29:50
使用范围: 科研使用
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Anti-Dopamine Receptor D1抗体
产品编号 | YB-10610R |
英文名称 | Dopamine Receptor D1 |
中文名称 | 多巴胺受体D1抗体 |
别 名 | DRD1; dopamine D1 receptor; D(1A) dopamine receptor; D1A dopamine receptor; Dopamine D1Receptors; D1DR; DADR; Dopamine Receptor D1; DR D1; DR D1A; DRD 1; DRD1 receptor; DRD1; DRD1A; DRD1_HUMAN; D(1A) dopamine receptor; DRD 1A; DRD1. |
规格价格 | 50ul/780元 购买 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
说 明 书 | 50ul 100ul 200ul |
研究领域 | 肿瘤 免疫学 神经生物学 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human, Rat, |
产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 50kDa |
细胞定位 | 细胞浆 细胞膜 |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human Dopamine Receptor D1:11-100/446 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
PubMed | PubMed |
产品介绍 | background: This gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene. [provided by RefSeq, Jul 2008] Function: Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase. Subunit: Interacts with DNAJC14 via its C-terminus. Interacts with DRD1IP. Subcellular Location: Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Tissue Specificity: Detected in caudate, nucleus accumbens and in the olfactory tubercle. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P21728 Gene ID: 1812 Database links: Entrez Gene: 1812 Human Entrez Gene: 13488 Mouse Entrez Gene: 24316 Rat Omim: 126449 Human SwissProt: P21728 Human SwissProt: Q61616 Mouse SwissProt: P18901 Rat Unigene: 2624 Human Unigene: 54161 Mouse Unigene: 24039 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品图片 |
Sample:A549 Cell(Human)Lysate at 40 ug
Primary: Anti-Dopamine Receptor D1(bs-10610R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50kD Observed band size: 60kD Sample:
A431 Cell(Human)Lysate at 40 ug HepG2 Cell (Human) Lysate at 40 ug Primary: Anti-Dopamine Receptor D1(bs-10610R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50kD Observed band size: 60kD Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Dopamine Receptor D1 Polyclonal Antibody, Unconjugated(bs-10610R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Cell: SH-SY5Y
Concentration:1:100 Host/Isotype:Rabbit/IgG Flow cytometric analysis of Rabbit IgG isotype control (Cat#: bs-10610R) on SH-SY5Y(green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody . |