SPECIFICATION SHEET

Catalog #: L1C01001 Lot #: C081201

Description: MAb to Cancer Antigen CA24-2
Monoclonal Antibody to Human Cancer Antigen CA24-2 (Gastrointestinal Cancer)

Specificity: Reacts specifically with Sialyl Lewisa containing glycolipids, without detectable reactivity with Lewisª, Lewisb or structurally related substances as determined by solid-phase binding assays against glycolipid antigens. Shows minimal cross-reactivity (<1%) with Sialyl Lacto-N-tetraosylceramide.

Clone: ML04C01(C242)

Host Animal: Mouse. Hybridization of Sp2/0 myeloma

Isotype: IgG1 cells with spleen cells from Balb/c mice.

Source: mouse ascites

Immunogen: Human colorectal adenocarcinoma cell-line COLO-205

Format: Purified, Liquid

Purification: Protein A chromatography

Concentration: 2.5mg/ml (OD280nm, E0.1% = 1.4)

Affinity Constant: 7 x 10-9 mol/l

Buffer: 0.01M PBS PH7.4

Preservative: None

Applications: Capture antibody in serological assays for the research of CA 24-2. Immunohistochemistry on frozen or paraffin sections for the detection of Sialyl Lewisa. Immunochemical detection of Sialyl Lewisa determinant in mucins and glycolipids. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Recommended antibody pair for immunoassay:
Capture Detection
L1C01001 L1C01002

QC Information: IgG >95% of total peak area (Superdex 200 gel chromatography on ÄktaFPLC).

Storage: Short-term (up to 2 months) store at <-20°C. Long term (>2 months) store at <–40°C.