Product: Anti-LT
R mAb, clone 4H8 WH2
Cat. No.: MC-566 (100 μg)
Synonyms:
Anti-Lymphotoxin-
Receptor; anti-Tumor
Necrosis Factor Receptor 2 related Protein; anti-
Tumor Necrosis Factor C Receptor; anti-
TNFRSF 3; anti-Tumor Necrosis Factor
Receptor Superfamily Member 3
Description:
The monoclonal antibody to mouse LT
R is an
agonist that can be used for the investigation of
the regulation of BAFF(BlyS), chemokines and
integrins using in vivo and tissue culture models,
the development of NK cells, and NK T cells, to
study the regulation of NF-
B family of
transcription factors in regulation of inflammation
and homeostasis, particularly ReIB NF-
B2
pathway.
Specificity:
Recognizes mouse LT
R
Species Reactivity:
Mouse. Other species not tested.
Isotype:
Rat IgG2a
Immunogen:
Recombinant mouse LT
R cysteine-rich region
(aa 31-221).
Format:
100 μg of liquid monoclonal antibody at 1 mg/mL
in PBS.
Purity:
98 as determined by SDS-PAGE.
Storage:
Store at 4°C short term and at -20°C long term.
Aliquot to avoid freeze/thaw cycles.
Applications:

Flow cytometry

Functional application: Agonist inducing
BAFF, chemokines and integrins in vitro and
in vivo. Add to cell cultures at 2 μg/mL. For
in vivo use, mice are injected
intraperitoneally with 50 μg/mL of antibody
clone 4H8 WH2 in sterile phosphate saline.
The researcher should determine the optimal
dilution for a specific application.
Figure: Flow cytometry analysis of endogenous LTbR on
mouse NIH3T3 cells by monoclonal antibody to mLTbR (MC-
566).
Method: NIH3T3 cells were stained with isotype control
(black line) or monoclonal antibody to LTbR (MC-566) (green
line) at 1 μg/mL, detected by goat anti rat IgG PE-conjugated
secondary antibody and analyzed by flow cytometry.
Limitations:
For research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product