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20 % off blocking (normal) serum and negative control

活动品牌:Agrisera|Serum and negative control | 有效期:2021.07.01-2021.09.30

20 % off blocking (normal) serum and negative control


20 % off Agrisera blocking (normal) serum

Agrisera offers a wide range of high-quality blocking (normal) serum for the application as a blocking agent, or as a negative control for immunoassays. All serum is obtained from healthy animals. The following formats are available:

  • Serum
  • Total immunoglobulin fraction
  • Highly purified, total immunoglobulin fraction
From the following species: Bovine | Chicken | Donkey | Duck | Goat | Guinea pig | Hamster | Horse | Human | Llama | Mouse | Pig | Rabbit | Rat | Sheep

Serum is lipid-extracted, dialyzed against phosphate buffer, and provided as lyophilized powder without preservative in different amounts:

From 1-10 mg to 1 g and bulk.

For most immunoassays like IHC, ELISA or Western blot, blocking is a crucial step to perform. This procedure blocks spaces that are not occupied by immobilized proteins, which prevents non-specific binding of the antibodies used in the assay. If blocking is not performed, false signals or high background will occur.

There is no universal blocking agent, therefore empirical testing is necessary to determine which agent to use for a given assay.

If normal serum is used as a blocker, it is recommended to use serum derived from the same host species as the detection antibody, conjugated with the reporter enzyme like HRP (Horse radish peroxidase) or ALP (Alkaline phosphatase).

Example:
Primary antibody: Rabbit anti-target

Blocking serum: Normal Chicken Serum (usually used at 5 % v/v in PBS with Tween®20 detergent)

Secondary antibody: Chicken anti-Rabbit IgG-HRP conjugated

Note: Rabbit serum cannot be used for blocking in such a combination.

Collection of discounted negative control reagents

Use discount code: Block20

Valid July-September 2021




2021-07-01 @ 12:45:55


靠谱
以上活动最终解释权归 Agrisera AB 所有

Agrisera AB   商家主页

地址:21 Vännäs, SWEDEN, Europe
联系人:Dr. Joanna Porankiewicz-Asplund
电话:0046704137117
传真:004693533044
Email:

日期 数量
2021-9 7
2021-10 1
2021-11 3
2021-12 2
2022-1 0
2022-2 0

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电话:021-54485309

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