HITRAP NHS-ACTIV HP
产品名称： HITRAP NHS-ACTIV HP
英文名称： HITRAP NHS-ACTIV HP
产品编号： 17-0716-01
产品价格： 0
产品产地： USA
品牌商标： 美国GE
更新时间： null
使用范围： null
上海基星生物科技有限公司
- 联系人 :
- 地址 : 上海市宝山区友谊路1506号东智广场1109室
- 邮编 : 201900
- 所在区域 : 上海
- 电话 : 138****5983
- 传真 : 021-50801884
- 邮箱 : 342786402@qq.com
| 17-0716-01 | HITRAP NHS-ACTIV HP, 5 X 1ML |
| 17-0716-30 | HiTrap NHS-activated HP, 180 x |
| 17-0717-01 | HITRAP NHS-ACTIV HP, 1 X 5 ML |
| 17-0920-03 | HITRAP IMAC HP, 5 X 1 ML |
| 17-0920-05 | HITRAP IMAC HP, 5 X 5 ML |
| 17-0921-02 | HITRAP IMAC FF, 5 X 1ML |
| 17-0921-04 | HITRAP IMAC FF, 5 X 5 ML |
| 17-0978-13 | HITRAP BUTYL-S FF, 5 X 1ML |
| 17-0978-14 | HITRAP BUTYL-S FF, 5 X 5ML |
| 17-1128-01 | MABTRAP KIT |
| 17-1151-01 | HITRAP SP HP, 5 X 1 ML |
| 17-1152-01 | HITRAP SP HP, 5X5 ML |
| 17-1153-01 | HITRAP Q HP, 5 X 1 ML |
| 17-1154-01 | HITRAP Q HP 5X5 ML |
| 17-1351-01 | HITRAP PHENYL HP, 5 X 1ML |
| 17-1353-01 | HITRAP PHENYL FF (LS), 5X1ML |
| 17-1355-01 | HITRAP PHENYL FF (HS),5 X 1ML |
| 17-1357-01 | HITRAP BUTYL FF, 5 X 1 ML |
| 17-1359-01 | HITRAP OCTYL FF, 5 X 1 ML |
| 17-1408-01 | HITRAP DESALTING, 5X5ML |
| 17-5053-01 | HITRAP Q FF, 5 X 1 ML |
| 17-5054-01 | HITRAP SP FF, 5 X 1 ML |
| 17-5055-01 | HITRAP DEAE FF 5 X 1 ML |
| 17-5056-01 | HITRAP CM FF, 5 X 1 ML |
HiTrap™ NHS-activated HP Columns
- Prepacked with NHS-activated Sepharose™ High Performance for coupling ligand via primary amines at near neutral pH.
- Simple coupling, carried out at close to neutral pH.
- Delivered in 100% isopropanol to protect the active groups.
HiTrap™ NHS-activated HP Columns
Technical Information
 calibration kit, reduced<para/>Lane 1: Desorbed material, non-reduced<para/>Lane 2: Low Molecular Weight (LMW) calibration kit, reduced'))
Lane 1: Desorbed material, reduced
Lane 2: Low Molecular Weight (LMW) calibration kit, reduced
Lane 1: Desorbed material, non-reduced
Lane 2: Low Molecular Weight (LMW) calibration kit, reduced
The use of NHS-activated Sepharose™ High Performance is illustrated by the purification of anti-mouse Fc-IgG from sheep antiserum after coupling mouse IgG to HiTrap™ NHS-activated. The contents of the different fractions were analysed by SDS-PAGE using PhastSystem™ and Goomassie staining.
| TECHNICAL SPECIFICATIONS | |
| Group to be coupled | -NH2 |
| Coupling conditions | pH 6.5-9 at 4°C to 25°C |
| Matrix | Highly cross-linked agarose, 6% |
| Substitution | 10 μmol NHS/ml medium |
| Spacer arm | 6-aminocaproic acid, 10-atom |
| pH stability* | 3-12 |
| Average particle size | 34 μm |
| Storage | 100% isopropanol |
| Storage temperature | 4°C to 8°C |
| * Ligand dependent.
|
|
For column specifications, see Prepacked HiTrap™ Affinity Columns.
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Purification of anti-mouse Fc-IgG from sheep antiserum.