HiScreen Capto IEX Columns

• Prepacked ready to use columns with different Capto IEX media for convenient process development optimization
• Ideal for parameter method optimization and parameter screening due to the 10 cm bed height
• Easy connection in series to achieve 20 cm bed height
• Small bed volume gives fast results and minimal sample/buffer consumption
• Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity

HiScreen Capto IEX Columns

Technical Information

HiScreen Capto IEX columns are part of the process development platform available from GE Healthcare. Five prepacked HiScreen columns with Capto Q, Capto DEAE (strong and weak anion exchange, respectively), Capto S (strong cation exchange), Capto adhere (strong multimodal anion exchange) and Capto MMC (weak multimodal cation exchange) are available.

These columns are due to the small column volume, 4.7 ml and 10 cm bed height excellent tools for method optimization, parameter screening, robustness testing and convenient scaling up. Process fluid velocities can be applied, since the 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale up.

If necessary, two columns can easily be connected in series to give a bed height of 20 cm.

Media prepacked in HiScreen Capto IEX columns are also available in other formats and as bulk packs, which makes it possible to use the same media for development work, pilot studies, and routine production.

HiScreen Capto IEX columns, prepacked for convenient and robust process development.

Robustness study with overlay of absorbance curves at 280 nm for cycles 1, 5 and 10 using HiScreen Capto MMC. The differences in UV signal is due to the use of different sample preparations.