HISTRAP FF CRUDE-色谱柱 -耗材-生物在线
HISTRAP FF CRUDE

HISTRAP FF CRUDE

商家询价

产品名称: HISTRAP FF CRUDE

英文名称: HISTRAP FF CRUDE

产品编号: 11-0004-58

产品价格: 0

产品产地: 美国

品牌商标: GE

更新时间: null

使用范围: null

上海基星生物科技有限公司
  • 联系人 :
  • 地址 : 上海市宝山区友谊路1506号东智广场1109室
  • 邮编 : 201900
  • 所在区域 : 上海
  • 电话 : 138****5983
  • 传真 : 021-50801884
  • 邮箱 : 342786402@qq.com

11-0003-29 HITRAP DESALTING 100 X 5 ML
11-0004-58 HISTRAP FF CRUDE 5X1 ML
11-0004-59 HISTRAP FF CRUDE 100X1 ML
11-0004-64 STOP PLUG FEMALE, 1/16''
11-0013-02 HITRAP CAPTO Q, 5X1ML
11-0013-03 HITRAP CAPTO Q, 5X5ML
11-0032-73 HITRAP CAPTO MMC, 5X1ML
11-0032-75 HITRAP CAPTO MMC, 5X5ML
11-0034-93 HITRAP MABSELECT SURE, 5X1ML
11-0034-94 HITRAP MABSELECT SURE, 1X5ML
11-0034-95 HITRAP MABSELECT SURE, 5X5ML
17-0402-01 HITRAP PROTEIN A HP, 5 X 1 ML
17-0402-03 HITRAP PROTEIN A HP,2 X 1 ML
17-0403-01 HITRAP PROTEIN A HP,1 X 5 ML
17-0403-03 HITRAP PROTEIN A HP, 5 X 5 ML
17-0404-01 HITRAP PROTEIN G HP, 5 X 1 ML
17-0404-03 HITRAP PROTEIN G HP, 2 X 1 ML
17-0405-01 HITRAP PROTEIN G HP,1 X 5 ML
17-0405-03 HITRAP PROTEIN G HP, 5 X 5 ML
17-0406-01 HITRAP HEPARIN HP, 5 X 1 ML
17-0407-01 HITRAP HEPARIN HP,1 X 5 ML
17-0407-03 HiTrap Heparin HP 5x5ml

HisTrap™ FF crude Columns/HisTrap FF crude Kit - Convenient Purification from Unclarified Lysates

  • Optimized for purification of histidine-tagged* proteins directly from homogenized, unclarified cell lysates.
  • Simply homogenize and load your sample directly.
  • High binding capacity, approx. 40 mg/ml medium and negligible Ni2+ leakage.
  • Compatible with a wide range of reducing agents, detergents, denaturants and other additives.
  • Convenient kit provides columns and buffers for quick and easy purifications.
  • Simple operation with a syringe, pump, or chromatographic system such as ÄKTAdesign.

HisTrap™ FF crude Columns/HisTrap FF crude Kit - Convenient Purification from Unclarified Lysates

Technical Information


* For licensing information, see Legal Info.

 

Customer feedback on Ni Sepharose 6 Fast Flow.

 

TECHNICAL SPECIFICATIONS
Medium  Ni Sepharose 6 Fast Flow 
Column volume  1 ml and 5 ml 
Column dimensions  0.7 × 2.5 cm (1 ml)
1.6 × 2.5 cm (5 ml) 
Dynamic binding capacity*  Approx. 40-mg histidine-tagged protein/ml medium 
Recommended flow rate  1 ml/min (1 ml)
5 ml/min (5 ml) 
Max. pressure  3 bar (0.3 MPa, 42 psi) 
Compatibility  during use  Stable in all commonly used buffers, reducing agents, denaturing agents and detergents, see Ni Sepharose™ High Performance - High-performance Purification 
Chemical stability  For one week at 40°C: 0.01 M HCl, 0.1 M NaOH
For 12 h: 1 M NaOH, 70% acetic acid
30 min tested: 30% 2-propanol
1 h tested: 2% SDS 
pH stability  2-14 (short term), 3-12 (long term) 
Storage  20% ethanol 
Storage temperature  +4°C to 30°C 
* Protein-dependent
† H2O at room temperature
‡ Ni2+-stripped medium

 

 

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HisTrap™ FF crude columns allow simple, one-step purification of histidine-tagged proteins without sample pretreatment such as centrifugation and filtration.

 

HisTrap™ FF crude 1-ml and 5-ml columns offer the convenience of simple, one-step purification of histidine-tagged proteins directly from homogenized, unclarified cell lysates. Direct loading of unclarified sample both saves time and prevents degradation of sensitive target protein.

 

HisTrap™ FF crude columns are prepacked with Ni Sepharose™ 6 Fast Flow. For more information see Ni Sepharose™ 6 Fast Flow - Scale-up Purification. For column technical specifications, see HisTrap™ FF Columns - Easy Scale-up Purification (HisTrap™ FF Columns). For best results, we recommend first addition of lysozyme and DNase I followed by a mechanical lysis, e.g. by sonication. The homogenized sample can then be loaded directly on the column without a clarification step which may prevent degradation of the target protein and increase the activity.

 

HisTrap FF crude Kit provides convenient purification of histidine-tagged proteins. The kit contains three HisTrap FF crude 1-ml columns, phosphate buffer, pH 7.4 (2 × 50 ml [8× concentrate]), 2 M imidazole, pH 7.4, all necessary connectors, a syringe, and instructions.

 

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Four repeated purifications of MBP-(His)6 from E. coli extract on HisTrap FF crude 1 ml. The medium was subjected to Ni2+-ion stripping, cleaning-in-place, and recharging between runs.

 

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HisTrap FF crude columns save time in the purification of histidine-tagged proteins compared to conventional IMAC.