- 时间: 2008/1/30
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Catalog No: KC-4O001
Size: 25 μg
Physical Appearance: Colorless oil
Molecular Formula: C44H68O13
Molecular Weight: 805.0
Purity: 98% (HPLC)
Solubility: Soluble in ethanol (25 mg/ml) and DMSO (25 mg/ml)
Storage: Freezer (-20℃). Protect from light. Following reconstitution, aliquot and freeze (-20℃). This product is stable for 2 years as supplied. Stock solutions are stable for up to 1 month at -20℃.
Description: An ionophore-like polyether derivative of a C38 fatty acid compound isolated from the dinoflagellate Prorocentrum sp. that has fed on the marine sponge Halinchrondria okadai. It is a potent non-comepetitive, reversible inhibitor of serine/threonine-specific protein phosphatases 1 (PP1, IC50 = 10-15 nM, rabbit skeletal muscle, catalytic subunit) and 2A (PP2A, IC50 = 0.1 nM, rabbit skeletal muscle, catalytic subunit). It has only a trivial effect on protein phosphatase 2B (IC50 = 5 μM), a Ca2+/calmodulin-dependent enzyme, while PP2C, a Mg2+ dependent enzyme, is unaffected. Okadaic acid has no significant effect on the activities of tyrosine phosphatases, alkaline phosphatase, acid phosphatase, and inositol trisphosphatase. It is also a non-phorbol ester-type tumor promoter on mouse skin. Useful for the study of protein phosphatases and of PP1/PP2A in cell extracts, as well as in intact cells. Induces apoptosis in human breast carcinoma (MB-231 and MCF-7) and in myeloid cells, but inhibits glucocorticoid-induced apoptosis in T-cell hybridomas. Has marked contractile effect on smooth muscles and heart muscles. Implicated as a causative agent of diarrhetic shellfish poisoning.
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CAUTION!!! Avoid all forms of contact with this product by wearing appropriate protective clothing (gloves, mask, lab coat). The Material Safety Data Sheet (MSDS), is available on request.