Sci Total Environ :湘潭大学丨城市污水中的季铵盐对绿藻NH4+吸收的影响(附NMT实验体系)

来源: 旭月(北京)科技有限公司   2019-10-18   访问量:265评论(0)

期刊:Science of The Total Environment

主题:城市污水中的季铵盐对绿藻NH4+吸收的影响

标题:Extracellular polymeric substrates of Chlorella vulgaris F1068 weaken stress of cetyltrimethyl ammonium chloride on ammonium uptake

影响因子:4.610

监测指标:NH4+流速

检测部位:小球藻藻细胞

NH4+流速流实验处理方法:

小球藻,有无0.5 mg/L CTAC在光照/黑暗下处理6小时

NH4+流速流实验测试液成份:
NH4Cl 37.4 mg/L, NaH2PO42·7H2O 9.8 mg/L, CaCl2·2H2O 14.7 mg/L,MgSO4·7H2O 24.6 mg/L and NaHCO3 42mg/L,pH 7.5

作者:湘潭大学葛飞、匡扬铎

英文摘要

This study investigated the influences of cetyltrimethyl trimethyl ammonium chloride (CTAC), an emerging pollutant quaternary ammonium compound (QAC) in municipal effluents, on the transfer and uptake of NH4+ by Chlorella vulgaris F1068 cells removed EPS artificially (EPS-R) and coated EPS naturally (EPS-C) under different scenarios (e.g., the presence or absence of CTAC, different photoperiod sequences (light 12 h: dark 12 h or dark 12 h: light 12 h)).

The results showed that the removal of EPS increased the transfer and uptake of NH4+ but the presence of EPS caged NH4+ and effectively weakened the stress of CTAC (<0.5 mg/L) on NH4+ uptake. The main mechanism was considered that CTAC in the concentration range from 0.1 to 0.5 mg/L induced an increased amount of polysaccharide and protein in EPS and thus protected algal normal physiological functions (including cell membrane permeability and glutamine synthetase activity) from the damage of CTAC (0.1 to 0.5 mg/L) regardless of the photoperiod sequences.

Thereby, the findings of this study provided an insight into the role of algal EPS in transfer and uptake of nutrients under the coexisted toxics for the future algae-based sewage treatment application.

中文摘要(谷歌机翻)

这项研究调查了十六烷基三甲基三甲基氯化铵(CTAC)(一种新兴的污染物季铵化合物(QAC))在城市污水中对寻常绿藻F1068细胞人工去除EPS(EPS-R)和包被EPS的转移和吸收NH4+的影响。自然地(EPS-C)在不同情况下(例如,是否存在CTAC,不同的光周期序列(亮12 h:暗12 h或暗12 h:亮12 h))。

结果表明,EPS的去除增加了NH4+的转移和吸收,但是EPS的存在笼罩了NH4+,有效地减弱了CTAC(<0.5μmg/ L)对NH4+吸收的胁迫。认为其主要机理是浓度在0.1至0.5μmg/ L的CTAC诱导EPS中的多糖和蛋白质增加,从而保护藻类的正常生理功能(包括细胞膜通透性和谷氨酰胺合成酶活性)免受CTAC的损害。 (0.1至0.5μmg/ L),与光周期无关。

因此,本研究的发现为藻类EPS在未来基于藻类的污水处理应用中在共存有毒物质的转移和吸收养分中的作用提供了见解。

结果表明:在没有CTAC的6小时光照下,EPS-C细胞或EPS-R细胞的NH4+通量分别达到-1.87ng /(cm2·s)和-2.63ng /(cm2·s)。当添加0.5 mg / L的CTAC时,EPS-C或EPS-R细胞的NH4+通量急剧下降至-0.94 ng /(cm2·s)和-0.73 ng /(cm2·s)。相反,当EPS-R细胞和EPS-C细胞在黑暗中培养6小时无论CTAC存在与否,EPS-C细胞和EPS-R细胞的NH4+流速从负值转换为正值,表明NH4+从细胞内环境流出到培养基。

文章链接:https://www.sciencedirect.com/science/article/pii/S0048969718353683?via%3Dihub



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