生物芯片 蛋白质芯片NEXTERION® HiSens Slides
产品名称: 生物芯片 蛋白质芯片NEXTERION® HiSens Slides
英文名称: NEXTERION® Reflective optical HiSens Slides
产品编号: 蛋白质芯片004
产品价格: 0
产品产地: 德国
品牌商标: SCHOTT NEXTERION®
更新时间: null
使用范围: null
- 联系人 :
- 地址 : 萝岗开发区科学城南云一路2号历康实业大楼3层
- 邮编 : 510663
- 所在区域 : 广东
- 电话 : 185****9007
- 传真 : 020-82510371
- 邮箱 : don.zeng@siooptics.com
应用于微阵列扫描仪、微阵列定制、蛋白质芯片、微阵列生物芯片、小分子微阵列、生物分子检测、抗体、全蛋白质组微阵列、三维凝胶块芯片、乙肝诊断蛋白质芯片、寡核苷酸微阵列、微孔板蛋白芯片、SARS 诊断蛋白质芯片、化学微阵列、信号检测分析、瘤诊断蛋白质芯片、基于细胞的微阵列、探针的制备、蛋白质芯片载体、寡糖微阵列、固体芯片的构建、蛋白质玻片、活细胞微阵列、疾病诊断、蛋白质微阵列、微阵列芯片扫描仪、药物筛选、抗原、同位素标记、生化反应的检测、抗体筛选、抗原抗体检测、基因表达的筛选、环氧基修饰蛋白质芯片、醛基修饰蛋白质芯片、3D蛋白质芯片、探针类型寡核苷酸、探针类型多肽等研究检查领域。
Reflective optical coating 反射光学镀膜
NEXTERION® HiSens Slides
- Only a proportion of the total excitation light is absorbed by the micro array spots, with most passing through the glass slide.
- On standard non-reflective slides, there is weak, but “destructive” interference of the excitation light waves, leading to a lower fluorescent emission.
- The light emitted by the fluorescent dyes attached to the target molecule is undirected and lost through the back of the transparent glass.
Overview
Type of coating | Ordering information | |||
NEXTERION®product | Barcode option | Item number | Slides per pack | |
Reflective optical coating available with most of NEXTERION® functional coatings | HiSens uncoated | None | 1141518 | 25 |
Label | 1141519 | 25 | ||
HiSens AStar | None | 1178045 | 25 | |
Label | 1178046 | 25 | ||
HiSens A+ | None | 1139673 | 25 | |
Label | 1141515 | 25 | ||
HiSens AL | None | 1139827 | 25 | |
Label | 1141514 | 25 | ||
HiSens E | None | 1125813 | 25 | |
Label | 1137332 | 25 | ||
HiSens H | None | 1141517 | 25 | |
Label | 1139829 | 25 | ||
HiSens P | None | 1178051 | 25 | |
Label | 1178052 | 25 |
Key product features |
• Significantly improved signal to background ratios for identifying low-expressor genes or low-abundant proteins |
• Easy implementation with standard slide protocols |
• Compatible with all common microarray equipment |
• Reduced target concentrations |
• Reduced reagents and dye costs |
• Preservation of Cy3/5™ ratios – comparable to data from conventional slides |
• Chemically stable reflective coating |
Immobilization chemistry
Suitable probe types |
The functional coating on NEXTERION® HiSens Slides is identical to the respective coating on NEXTERION® Slides. Please refer to the table in the section “General information coated slides” in order to find the appropriate coating chemistry for your specific application. |
Product details
The NEXTERION® HiSens coating is able to enhance signal amplification by exploiting several optical effects:
- Fluorescence enhancement and reflection of excitation light
On a standard glass microarray slide, the fluorescently labelled spots absorb only some of the excitation light. By placing a dielectric coating beneath the spots, this “lost” light is reflected back onto the spots. In addition, the multiple layers of the HiSens coating have been specifically designed so that the incoming and reflected excitation light waves overlap and interfere with each other. The interference is constructive at the slide surface, enhancing the excitation by a theoretical factor of up to 6.3 times.
- Reflection of the emission light.
- The fluorescence emission in the Cy3™ and Cy5™ channels from the labelled spots is redirected towards the detector rather than being lost by passing through the glass or by scattering. This effectively doubles the detected fluorescence signal.
Taken together, these effects can theoretically enhance the fluorescence signal by up to 12.6 times when compared to standard transparent microarray slides.
Increased sensitivity
Significant increases in fluorescent signal intensities and signal-to-background ratios have been demonstrated with the NEXTERION® HiSens E and A+ products, the first two slide surfaces launched by SCHOTT in the HiSens range in 2006 (all slide surfaces are now available). The functional coatings are identical to the standard NEXTERION® slides and were processed with standard slide processing protocols. The results clearly exhibit the same impressive spot size and morphology that users typically associate with NEXTERION® Slide E and Slide A+, but the signal intensity is significantly enhanced thanks to the HiSens coating.
Identical oligonucleotide probes were printed on NEXTERION® HiSens A+ and the standard NEXTERION® Slide A+. The slides were hybridized and scanned on an Axon 4000B scanner with identical settings. Images of the NEXTERION® HiSens reflective coating are compared to standard Slide A+. The NEXTERION® HiSens A+ data was normalized against the standard NEXTERION® Slide A+. An 8 to 10 fold increase in signal-to-background ratios was obtained with the NEXTERION®HiSens reflective coating compared to the standard NEXTERION® Slide A+.
NEXTERION® HiSens is the ultimate microarray slide for microarray applications where the target material is at a low concentration or cannot be reliably amplified, for example with mRNAs or low abundant proteins. The NEXTERION® HiSens coating may be used to produce reproducible signals at a much lower target concentration than with conventional slides.
Preservation of dye ratios
The reflective layers in the NEXTERION® HiSens coating have been designed to enhance the performance of both the Cy3™ and Cy5™ channels equally. The Cy3™ to Cy5™ ratios are preserved, allowing use of standard data normalization methods and direct comparison with data previously produced on conventional slides.