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Alternate Method for Purifying DNA from Agarose Gels

来源:本站原创  2006/7/29 6:46:00   访问量:6282
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1. Excise band of interest from a TAE gel; estimate volume by weighing the gel slice.

2. Dissolve the gel slice in 3 volumes of NaI (from Geneclean kit) at 55EC for 5 min or until the gel dissolves.

3. Add 1 ml of Wizard miniprep resin, 10 min. at room temperature.

4. Add DNA resin mixture to a syringe and pass through a minicolumn.

5. Wash with 2 ml of Wizard wash buffer; spin 2 min in a microfuge.

6. Elute with 70EC TE or water (50 μl), 2 min in a microfuge.

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