Human Growth Factors & Cytokines
Recombinant Human NOGGIN





 





		Synonyms :
none



		Description :
Noggin belongs to a group of diffusible proteins which bind to ligands of the TGF-β family and regulate their activity by inhibiting their access to signaling receptors.  The interplay between TGF-β ligands and their natural antagonists has major biological significance during development processes, in which cellular response can vary considerably depending upon the local concentration of the signaling molecule.  Noggin was originally identified as a BMP-4 antagonist whose action is critical for proper formation of the head and other dorsal structures.  Consequently, Noggin has been shown to modulate the activities of other BMPs including BMP-2,-7,-13, and -14.  Targeted deletion of Noggin in mice results in prenatal death and recessive phenotype displaying a severely malformed skeletal system.  Conversely, transgenic mice over-expressing Noggin in mature osteoblasts display impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis.  Recombinant human Noggin is a 46 kDa disulfide-linked homodimer (120-10C) consisting of two 206 amino acid polypeptide chains.  Monomeric glycosylated Noggin migrates at an apparent molecular weight of approximately 28.0-33.0 kDa by SDS PAGE analysis under reducing conditions.



		Catalog #:
120-10C



		Source :
293 cells



		Purity :
Greater than 95% by SDS-PAGE gel and HPLC analyses.



		Endotoxin Level :
Endotoxin level is less than 0.1 ng per μg (1EU/μg).



		Biological Activity :
Determined by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC chondrogenic cells.  The expected ED50 for this effect is 2.0-3.0 ng/ml of Noggin.


Selected References :
Selected List of References Citing This PeproTech Product



                AA Sequence :
QHYLHIRPAP SDNLPLVDLI EHPDPIFDPK EKDLNETLLR SLLGGHYDPG FMATSPPEDR PGGGGGAAGG AEDLAELDQL LRQRPSGAMP SEIKGLEFSE GLAQGKKQRL SKKLRRKLQM WLWSQTFCPV LYAWNDLGSR FWPRYVKVGS CFSKRSCSVP EGMVCKPSKS VHLTVLRWRC QRRGGQRCGW IPIQYPIISE CKCSC 



		PubMed Link :
NOGGIN


Country of Origin :
USA
















        Size A
5μg
$80 
 


 



        Size B
20μg
$195 
 


 



        Size C
1mg
$3900 
 


 















Special 
                    Sizes: 
 Many products can be ordered in custom sizes. Please click here to contact Peprotech with your request.





Usage:
For Research Use Only.  Not for use in diagnostic or therapeutic procedures.