淋巴系统是免疫系统的重要组成部分。维持体内稳定，它属于血管系统，但也有其独立的功能。淋巴系统循环使液体和大分子从组织回到血液。在调节体液、蛋白和组织压力平衡等方面有着重要的作用。虽然毛细淋巴管内皮细胞与血管内皮细胞有多种的共同特点，但其特殊的结构特点表现其特殊功能。毛细淋巴管缺少壁层细胞，表现为基底膜不完整或缺失。淋巴管内皮细胞特征有内陷、胞质囊和重叠的细胞间连接。毛细淋巴管最显著的特征之一就是它的整合作用，即通过弹性纤维的丝条连接细胞外基质。

    人表皮淋巴内皮细胞(HDLEC)提取于人真皮组织，原代冻存。每管含有细胞数>5×10⁵ cells/ml，此细胞通过Podoplanin , Lyve1. 和CD31免疫荧光染色验证，经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。细胞可以达到15倍增。

推荐培养基:(ECM, Cat. No. 1001)

Description

The lymphatic system is an essential part of the immune system. It serves distinct yet complementary function with the blood vascular system in maintaining tissue homeostasis. The lymphatic system returns fluid and macromolecules from the tissues back into blood circulation and, thus, plays a vital role in the regulation of fluid, protein, and pressure equilibrium in tissues [1]. Although lymphatic capillary endothelial cells have many properties in common with the endothelium of blood vessels, they also have distinct structural characteristics reflecting their specific functions. Lymphatic capillaries lack mural cells and are characterized by an incomplete or absent basement membrane [2]. Lymphatic endothelium typically contains numerous invaginations and cytoplasmic vesicles as well as characteristic overlapping intercellular junctions [3]. One of the most striking characteristics of the lymphatic capillary is its integration within the interstitium which is connected to the extracellular matrix by fine strands of elastic fibers.

HDLEC from ScienCell Research Laboratories are isolated from human dermal tissue. HDLEC are cryopreserved on passage two after purification and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HDLEC are characterized by immunofluorescent method with antibodies to CD31, podoplanin and Lyve1. HDLEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HDLEC are guaranteed to further expand for 15 population doublings at the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Endothelial Cell Medium (ECM, Cat. No. 1001) for the culturing of HDLEC in vitro.

Product Use

HDLEC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Shipping

Dry ice.

Reference

[1] Swartz, A. and Skobe, M. (2001) Lymphatic function, lymphangiogenesis, and cancer metastasis. Microsc. Res. Tech. 55:92-99.
[2] Leak, L. V. (1970) Electron microscopic observations on lymphatic capillaries and the structural components of the connective tissue-lymph interface. Microvasc. Res. 2, 361-391
[3] Podgrabinska, S., Braun, P., Velasco, P., Kloos, B., Pepper, M., Jackson, D. and Skobe, M. (2002) Molecular characterization of lymphatic endothelial cells. PNAS 99:16069-16074.