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上海中乔新舟生物科技有限公司是美国Sciencell公司中国一级代理：
美国ScienCell研究实验室（www.sciencellonline.com）成立于1999年，公司总部位于美国加州的圣地亚哥。主要致力于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发，在全球拥有众多客户。在国内销售15年来，很多老师应用其产品发表了高质量的SCI文章，且有着极高的文献引用率。凭借着严格的质控和优秀的产品品质，深受广大科研工作者的信赖。
ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制，细胞纯度可达98％。其中包括21种人体正常细胞系统，90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离，产品质量过硬。确保了实验结果的重复性和连贯性。
请登录Sciencell公司官方网站（www.zqxzbio.com或www.sciencellonline.com）以确保购买正规公司产品。
 
人冠状动脉内皮细胞说明：
货号：6020
Description
Endothelial cells constitute the natural interface between the blood and the underlying tissue. Changes in endothelial cell function appear to play a key role in the pathogenesis of atherosclerosis. Endothelial cells synthesize and secrete activators as well as inhibitors of both the coagulation system and the fibrinolysis system in addition to mediators that influence the adhesion and aggregation of blood platelets. Endothelial cells also release molecules that control cell proliferation and modulate vessel wall tone [1]. Human endothelial cells produce antithrombotic and thrombotic factors such as t-PA and PAI-1 and respond to TNF-alpha by modifying growth characteristics, producing cytokines such as GM-CSF, expressing ICAM-1 on the surface and producing large amounts of nitric oxide and endothelin [2]. Endothelial injury, with consequent endothelial dysfunction, is caused by percutaneous transluminal coronary angioplasty [3] and may play an important role in subsequent restenosis [4]. Many of the endothelial processes can be studied in vitro using cultured cells, and HCAEC will surely be very useful to studying and understanding the molecular mechanisms involved in coronary artery diseases.
HCAEC from ScienCell Research Laboratories are isolated from human coronary arteries. HCAEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HCAEC are characterized by immunofluorescent method with antibodies to vWF/Factor VIII and CD31 (P-CAM) and by uptake of DiI-Ac-LDL. HCAEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCAEC are guaranteed to further expand for 15 population doublings at the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Endothelial Cell Medium (ECM, Cat. No. 1001) for the culturing of HCAEC in vitro.
Product Use
HCAEC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Shipping
Dry ice.
Reference
[1] Elhadj, S. and Forsten, K. E. (2001) Chronic shear stress affects bovine aortic endothelial cell secreted proteoglycan production in vitro. Bioengineering Conference, Vol. 50:767-768.
[2] Donnini, D., Perrella, G., Stel, G., Ambesi-Impiombato, F. S., Curcio, F. (2000) A new model of human aortic endothelial cells in vitro. Biochimie 82:1107-14.
[3] Vassanelli C, Menegatti G, Zanolla L, Molinari J, Zanotto G, Zardini P. (1994) Coronary vasoconstriction in response to acetylcholine after balloon angioplasty: possible role of endothelial dysfunction. Coron Artery Dis. 5:979-986.
[4] Meurice T, Vallet B, Bauters C, Dupuis B, Lablanche JM, Bertrand ME. (1996) Role of endothelial cells in restenosis after coronary angioplasty. Fundam Clin Pharmacol. 10:234-242.
14.心脏细胞系统
6000 人心脏微血管内皮细胞 (HCMEC)( 5×105 )
6100 人主动脉内皮细胞 (HAEC)( 5×105 )
6110 人主动脉平滑肌细胞 (HASMC)( 5×105 )
6200 人心肌细胞 (HCM) (1×106 )
6210 成人心肌细胞 (HCMa) (1×106 )
6300 人心脏成纤维细胞( HCF) ( 5×105 )
6310 成人心室成纤维细胞 (HCFav)( 5×105 )
6320 成人心房成纤维细胞 (HCFaa)( 5×105 )
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