DIG Oligonucleotide Tailing Kit, 2nd generation：Formulation containing recombinant Terminal Transferase Kit for 25 tailing reactions of 100 pmol oligonucleotides
 
 
 
产品号
 产品规格
 厂商
 价格
 
03353583910
(3353583)
 1 kit (25 tailing reactions)
 Roche
 5335
 

 

Application

Oligonucleotides, tailed with DIG-dUTP, are used for DNA or RNA hybridization in membrane blots or in situ hybridization. 

Benefits

Very sensitive hybridization probes, due to the incorporation of several DIG-nucleotides Fast hybridization kinetics, due to the small size of oligonucleotides Single-stranded probes, no renaturation during hybridization Sequence can be designed according to the experiment Specially suited for in situ hybridization; due to their small size, oligonucleotides readily diffuse into fixed tissues and cells  Product Description

Tailing of oligonucleotides at the 3'-end with DIG-11-dUTP and recombinant Terminal Transferase. Oligonucleotides are tailed with DIG-dUTP and dATP at an average tail length of 50 nucleotides (tail length range: 10  100). 

Contents

1.       Reaction Buffer, 5x, 100 l 

2.       CoCl2 Solution, 100 l, 25 mM 

3.       DIG-dUTP Solution, 25 l, 1 mM 

4.       dATP Solution, 25 l, 10 mM 

5.       Recombinant Terminal Transferase, 25 l, [400 U/l] 

6.       Control Oligonucleotide, unlabeled, 25 l, [20 pmol/l] 

7.       Oligonucleotide, DIG-dUTP/dATP tailed, 20 l, [2.5 pmol/l] 

8.       Control DNA, 20 l, [0.25 mg/ml] 

9.       Glycogen Solution, 50 l, [20 mg/ml] 

10.    DNA Dilution Buffer, 3 x 1 ml, [50 g/ml] fish sperm DNA 

11.    poly(A) Solution, 1 ml, [10 mg/ml]  

Principle

DIG-dUTP and dATP are combined at a concentration that gives the highest DIG incorporation into the tail, and optimal spacing of DIG and dATP, to achieve the highest sensitivity in hybridization experiments. DIG-dUTP and dATP are provided as separate solutions to allow greater flexibility in terms of tail length, hapten spacing, and the use of unlabeled nucleotide(s).