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         ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制，细胞纯度可达98％。其中包括21种人体正常细胞系统，90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离，产品质量过硬。确保了实验结果的重复性和连贯性。

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人间充质干细胞--骨髓（Human Mesenchymal Stem Cells-bone marrow）

货号：7500

        间充质干细胞在成人的干细胞中数量恒定。它们有发育为成熟细胞的潜在能力而产生脂肪、软骨、骨骼和肌肉。这些特性与它们发育中的可塑性共同作用使人们对用间充质干细胞来替换受损组织的潜在能力产生了极大的兴趣。间充质干细胞培养在含有转化生长因子，无血清的培养基中将分化成关节软骨细胞，然而间充质干细胞培养在含血清，维C和地塞米松的培养基中将分化成造骨细胞。  间充质干细胞有更新和分化成不同间质组织系的能力。间充质干细胞在本质上讲可以通过培养来增加数量然后移植到受损部位或种在拟生态支架中以产生合适的组织形状。

Description

Mesenchymal stem cells (MSC) are well-characterized population of adult stem cells. They have the potential to develop into mature cells that produce fat, cartilage, bone, tendons, and muscle [1, 2]. These properties in combination with their developmental plasticity have generated tremendous interest in the potential use of mesenchymal stem cells to replace damaged tissues. MSC cultured without serum in the presence of transformation growth factor will differentiate into chondrocytes, whereas MSC cultured in serum with ascorbic acid and dexamethasone will differentiate into osteoblasts. MSC has the capability for renewal and differentiation into various lineages of mesenchymal tissues. In essence MSC could be cultured to expand their numbers then transplanted to the injured site or after seeding in/on shaped biomimetic scaffold to generate appropriate tissue constructs.

HMSC-bm from ScienCell Research Laboratories are isolated from human bone marrow. HMSC-bm are cryopreserved at passage one culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HMSC-bm are characterized by immunofluorescent method with antibodies to CD73, CD90, CD105 and lipid staining after differentiation. HMSC-bm are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HMSC-bm are guaranteed to further culture at the conditions provided by ScienCell Research Laboratories.

Recommended Medium
It is recommended to use Mesenchymal Stem Cell Medium (MSCM, Cat. No. 7501) for the culturing of HMSC-bm in vitro.

Product Use
HMSC-bm are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.

Shipping
Dry ice.

Reference
1. Kassem, M. Mesenchymal stem cells: biological characteristics and potential clinical applications. 2004. Cloning Stem Cells. 6(4):369-74.
2. Barry, F. P., and J. M. Murphy. Mesenchymal stem cells: clinical applications and biological characterization. 2004. Int J Biochem Cell Biol. 36(4):568-84.

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