鬼笔环肽 蓝色荧光上海翊圣生物科技有限公司

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产品名称: 鬼笔环肽 蓝色荧光
英文名称: Acti-stain 670 phalloidin
产品货号: PHDN1-A
产品规格: 500U
产品产地: cytoskeleton
产品商标: cytoskeleton
价    格: 2230元
产品资料: 实验方法技术资料
更新时间: 2019/10/10 9:30:00
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使用范围:仅限科研使用,不能应用于临床
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供应商联系卡

上海翊圣生物科技有限公司
地址:
上海市浦东新区天雄路166弄一号楼三层南单元
邮编:
200030
电话:
400-6111-883、021-34615995
传真:
021-34615995-188
联系人:
钟珍
所在区域:
上海·浦东新区
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公司展台:
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产品详细描述

 Acti-stain 670 phalloidin 鬼笔环肽 蓝色荧光

Product Uses Include

·         Fluorescent staining of fixed actin filaments in tissue sections and tissue culture cell preparations.Note: Unlike many actin antibodies, Acti-stain 670 phalloidin binds only to F-actin resulting in low background fluorescence. Furthermore, actin staining is not appreciably different between species.

·         Preparation of stabilized fluorescent actin filaments in vitro.

Actin staining is very useful in determining the structure and function of the cytoskeleton in living and fixed cells. The actin cytoskeleton is a very dynamic and labile structure in the living cell, but it can be fixed with paraformaldehyde prior to probing or staining for actin structures.

Material

Phalloidin is a seven amino acid peptide toxin from the mushroom Amanita phalloides, which binds specifically and with high affinity (Kd 20 nM) to the polymerized form of actin (F-actin). Phalloidin lowers the critical concentration of actin polymerization to less than 1 μg/ml, thereby acting as a polymerization enhancer. Phalloidin has been labeled with a proprietary far-red fluorescent dye which allows it to be used to stain actin filaments in tissue cultured cells and tissue sections (1, see Figure 1)and cell-free preparations. Acti-stain 670 phalloidin-labeled actin filaments retain many functional characteristics of unlabeled actin including their ability to interact with myosin. Actin-stain 670 phalloidin is supplied as a red solid.

Note: Phalloidin is toxic and must be handled with care (LD50 human = 2mg/Kg).


Example Results and Specifications

 

Figure 1. Actin Stress Fibers stained with Acti-stain™ 670 in a Swiss 3T3 cell.

 

Figure 2. Emission and excitation scans for Acti-stain™ phalloidins

 

 

 

 

 

Mitotic Swiss 3T3 cell, F-actin stained with Acti-stain™ 670 (far-red, Cat. # PHDN1), nuclear DNA stained with Dapi (blue).

 

Absorbance and fluorescence scan of Acti-stain™ 670. Labeled phalloidin was diluted into methanol and its absorbance and excitation spectra were scanned between 300-750 and 600-750 nm, respectively. Absorbance peaks at 625 nm and fluorescence at 675 nm.

 

Acti-stain phalloidins are the most well characterized phalloidins available. Tabe 1 describes their brightness, photostability, background and affinity constants to F-actin. Compare these performance characteristics to other fluorescent phalloidins and you will see the advantages of using Acti-stain for your actin staining requirements.

Table 1. Biochemical characteristics of fluorescent phalloidins

Conjugate

Cat.#

Wavelengths (Ex/Em)

Brightness (AFU*)

Stability to photobleaching(half life in seconds**)

Background (% of total AFU at 100nM**)

Affinity (Kd in nanomolar)

Fluorescein-phalloidin

na

485/535 FITC filter set

432

6

22

72 +/-12

Acti-stain™ 488

PHDG1

485/535 FITC filter set

832

57

5

55 +/-8

Acti-stain™ 535

PHDR1

535/585 TRITC filter set

430

27

12

36 +/-7

Acti-stain™ 555

PHDH1

535/585 TRITC filter set

551

46

16

63 +/-8

Acti-stain™ 670

PHDN1

640/680 Cy5 filter set

332

8

18

50 +/-12

* = AFUs measured by quantitative cell imaging. ** = Measured in stained Swiss 3T3 cells in the absence of antifade.

 

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生物在线声明:以上所展示的信息由企业自行提供,内容的真实性、准确性和合法性由发布企业负责。生物在线对此不承担任何保证责任。

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