The KAPA HTP Library Preparation Kit is designed for highthroughput
library construction for Illumina® sequencing,
starting from fragmented, double-stranded DNA. The kit
provides all of the enzymes and reaction buffers required
for the following steps of library construction:
1. End repair, which produces blunt-ended, 5-phosphorylated
fragments.
2. A-tailing, during which dAMP is added to the 3-ends
of blunt-ended dsDNA library fragments.
3. Adapter ligation, during which dsDNA adapters with
3-dTMP overhangs are ligated to 3-A-tailed library
fragments.
4. Library amplification (optional), which employs PCR
to amplify library fragments carrying appropriate
adapter sequences on both ends.

Product Applications
The KAPA HTP Library Preparation Kit is ideally suited
for high-throughput NGS library construction workflows
that involve end repair, A-tailing, adapter ligation, and
library amplification (optional). The kit has been tailored
to accommodate the specific requirements of automated
liquid handling, and the protocol may be adapted for
incorporation into workflows for a wide range of NGS
applications, including:
• Whole-genome shotgun sequencing
• Targeted sequencing by solution hybrid selection
(i.e. exome or custom capture using the Roche
Nimblegen™, Agilent SureSelect, Illumina® TruSeq™, or
IDT xGen™ Lockdown™ Probes systems)
• ChIP-seq
• RNA-seq
• Methyl-seq (in combination with the KAPA HiFi HotStart
Uracil+ ReadyMix)Library Amplification ReadyMix)

KAPA HTP Library Preparation Kit操作说明书.pdf