粪便DNA提取试剂盒-生物分子-试剂-生物在线
粪便DNA提取试剂盒

粪便DNA提取试剂盒

商家询价

产品名称: 粪便DNA提取试剂盒

英文名称: FastDNA Spin Kit For Feces

产品编号: 116570200

产品价格: 0

产品产地: USA

品牌商标: MP Biomedicals

更新时间: 2023-08-16T10:47

使用范围: null

北京智杰方远科技有限公司
  • 联系人 :
  • 地址 : 北京市海淀区马连洼北路九号东居兴业写字楼
  • 邮编 : 100196
  • 所在区域 : 北京
  • 电话 : 158****0961
  • 传真 : 010-62894435
  • 邮箱 : 15810200961@163.com

从人类和动物的粪便样品中有效分离基因组DNA,直接用于PCR

1.多达500mg的粪便样品

2.内含配合FastPrep仪 器使用的理解介质E

3.通常可以从500mg的粪便样品中分离得到10-20μg总DNA

4.去除对下游实验有影响的

快速高效提取粪便DNA,可裂解任何粪便样品。可直接用于PCR等其下游实验,配合FastPrep仪器使用无需研磨样品。

The FastDNA® SPIN Kit for Feces Designed for use with the FastPrep® Instruments, host cells as well as bacteria, fungi, viruses, protists and other cells present in fecal samples are easily lysed within 40 seconds. These benchtop devices use a unique, optimized motion that provide an extremely quick, efficient and highly reproducible homogenization that surpasses traditional extraction methods.

Samples are placed into 2.0 mL tubes containing Lysing Matrix E, a mixture of ceramic and silica particles designed to efficiently lyse all stool organisms. Stool samples are pre-treated by washing in a Pre-Lysis Buffer. Homogenization takes place in the presence of MT Buffer and Sodium Phosphate Buffer. The pre-lysing and lysing reagents were carefully developed to protect and solubilize nucleic acids upon cell lysis, facilitate extraction of genomic DNA , and minimize RNA, humic acid or polyphenol contamination. Following lysis, samples are centrifuged to pellet debris and lysing matrix. DNA is purified from the supernatant with a silica-based GENECLEAN® procedure using SPIN filters. Eluted DNA is ready for digestion, electrophoresis, PCR and any other desired application.

产品应用,如下:

The Fast DNA® SPIN Kit for Feces quickly and efficiently isolates PCR-ready genomic DNA from fresh or frozen human and animal stool samples.

产品属性,如下:

Product Type

Kits & Assays

Components

Lysing Matrix E 50x   2.0 mL tubes, PLS Solution 25 mL, MT Buffer 8 mL, Sodium Phosphate Buffer 100   mL, PPS 25 mL, Binding Matrix 66 mL, Wash Buffer #1 120 mL, Wash Buffer #2 12   mL, TES 20 mL, BBS Gel Loading Dye 200 μl, SPIN Filter Tubes 50 each, Catch Tubes 50 each

Instrument   Compatibility

FastPrep-24; FastPrep   120; FastPrep-96; SuperFastPrep-1

Sample Type

Soil; Feces;   Environmental Water; Wastewater; Sludge

Sample Volume

up to 500 mg per prep

Tube Compatibility

2 mL

Matrix Type

E

Molecule Enriched

DNA