riboFECT™ CP转染试剂-克隆与表达-试剂-生物在线
riboFECT™ CP转染试剂

riboFECT™ CP转染试剂

商家询价

产品名称: riboFECT™ CP转染试剂

英文名称: ribo FECT ™ CP Transfection

产品编号: C10511-05、C10511-1

产品价格: 0

产品产地: 广州

品牌商标: ribobio

更新时间: null

使用范围: null

广州市锐博生物科技有限公司
  • 联系人 :
  • 地址 : 广州科学城科学大道182号· 创新大厦C3栋 13层
  • 邮编 : 510663
  • 所在区域 : 广东
  • 电话 : 020-322*0221
  • 传真 : 020-32290137
  • 邮箱 : marketing@ribobio.com

riboFECT™ CP转染试剂是一种专门用于核酸分子的转染试剂,极具生物相容性,转染效率高,细胞毒性小,适合各种核酸分子(如siRNA、miRNA、质粒)的转染实验。该转染试剂转染时无需进行培养基更换操作,尤其适合各种高通量细胞筛选实验,方便,快速,高效

产品编号 产品名称 价格
C10511-05 ribo FECT ™ CP Transfection Kit (166T) ¥950
C10511-1 ribo FECT ™ CP Transfection Kit (333T) ¥1780

 

 

产品描述

riboFECT™ CP转染试剂是一种专门用于核酸分子的转染试剂,极具生物相容性,转染效率高,细胞毒性小,适合各种核酸分子(如siRNA、miRNA、质粒)的转染实验。该转染试剂转染时无需进行培养基更换操作,尤其适合各种高通量细胞筛选实验,方便,快速,高效

 

 

产品特点
 高转染效率,适用于大多数细胞的转染实验

■ 低细胞毒性,助您获得可靠的生理学数据

■ 操作简单方便,可反向转染,适合高通量筛选实验

 

-----高转染效率,低细胞毒性,只为更佳的基因沉默效率

      转染效率对基因沉默实验的重要性是毋庸置疑的。riboFECT  CP 转染试剂极具生物相容性,能够显著提高siRNA,miRNA的转染效率,为成功进行RNAi实验提供保证,并能够降低各种核酸分子最佳使用浓度,目前已 成功应用于各种真核细胞系的核酸转染,如HeLa,A549,293T,NIH/3T3,HepG2,HUVEC等细胞系。

                             1  分别用riboFECT™ CP转染HUVEC(A)和Hela(B)细胞,采用riboMONITOR检测转染效率

 

2 riboFECT™ CPReagent L分别转染p65 siRNAHUVECHela细胞,采用qPCR方法检测其基因沉默效率

结果显示riboFECT  CP转染效果更好,且随着转染试剂用量升高,转染效果越好,基因沉默效率也进一步提高


------操作流程简单,可反向转染,非常适合高通量筛选研究

仅需简单地将siRNAmiRNAbuffer混合后,加入riboFECT CP形成复合物,室温孵育0~10分钟,再加入一定体积的培养液,随后加入完全细胞培养基中即可,中途无需更换培养基

反向转染实验中,先将siRNAmiRNAriboFECT™ CP混合,加至培养孔以形成转染复合物,再将细胞加入至培养孔中进行反向转染,以实现细胞高通量筛选。其中,转染混合物制备后可保存较长时间,即允许提前准备和储存,非常适合自动化高通量筛选系统。


3 riboFECT™ CP转染操作简便,适合进行反向转染,且混合物制备后可稳定储存一段时间

 


------可高效转染质粒,尤其适合质粒共转实验

仅需少量的riboFECT CP即可实现质粒的高效转染,低毒性,对细胞伤害小,对大多数细胞转染均无需更换培养液,尤其适合进行质粒共转实验,例如miRNA靶基因双荧光素酶检测实验。

4 采用1µl riboFECT CP分别转染EGFP-C1A)和EGFP-N1B)于Hela细胞,24h后检测其表达效率

 

实用案例

转染试剂相关客户文章

编号

文章

杂志

转染细胞类型


MicroRNA-19b promotes macrophage cholesterol accumulation and aortic atherosclerosis by targeting ATP-binding cassette transporter A1

Atherosclerosis

HEK293T

1

Let-7a inhibits migration, invasion and epithelial-mesenchymal transition by targeting HMGA2 in nasopharyngeal carcinoma

Journal of Translational Medicine

CNE-2、5-8F

3

Erythropoietin attenuates advanced glycation endproducts-induced toxicity of Schwann cells in vitro.

Neurochem Res

SC


MicroRNA-145 contributes to enhancing radiosensitivity of cervical cancer cells

FEBS letters

HeLa


The microRNAs in the pathogenesis of metabolic memory

Endocrinology

HAEC


The expression of vesicular glutamate transporter 3 and vesicular monoamine transporter 2 induced by brain-derived neurotrophic factor in dorsal root ganglion neurons in vitro

Brain research bulletin

DRG


MicroRNA-101 protects cardiac fibroblasts from hypoxia-induced apoptosis via inhibition of the TGF-β signaling pathway

The International Journal of Biochemistry & Cell Biology

CF


Diosgenin inhibits atherosclerosis via suppressing the MiR-19b-induced downregulation of ATP-binding cassette transporter A1

Atherosclerosis

THP-1/MPM-derived foam cells


Integrated analysis of differential miRNA and mRNA expression profiles in human radioresistant and radiosensitive nasopharyngeal carcinoma cells

PloS one

CNE2-IR

3

Proteomic and bioinformatic analysis of differentially expressed proteins in denervated skeletal muscle

Int J Mol Med

L6 skeletal muscle cells


MiR-193a-3p promotes the multi-chemoresistance of bladder cancer by targeting the HOXC9 gene

Cancer Lett.

5637、H-bc

4

MiR-9a-5p regulates proliferation and migration of hepatic stellate cells under pressure through inhibition of Sirt1

World J Gastroenterol.

HSC

5

TRAF6 inhibition rescues dexamethasone-induced muscle atrophy

International Journal of Molecular Science

C2C12

6

Sodium fluoride induces apoptosis through reactive oxygen species-mediated endoplasmic reticulum stress pathway in Sertoli cells

Chinese Academy of Sciences

Sertoli

7

Fluid Shear Stress Upregulates E-Tmod41 via miR-23b-3p and Contributes to F-Actin Cytoskeleton Remodeling during Erythropoiesis

PloS one

MEL

8

KITLG is a novel target of miR-34c that is associated with the inhibition of growth and invasion in colorectal cancer cells

J Cell Mol Med.

HCT-116


MicroRNA-21 Negatively Regulates Treg Cells Through a TGF-β1/Smad-Independent Pathway in Patients with Coronary Heart Disease

Cell Physiol Biochem

PBMC

10

miR-193a-3p regulates the multi-drug resistance of bladder cancer by targeting the LOXL4 gene and the Oxidative Stress pathway

Mol Cancer

5637、H-bc


The DNA methylation-regulated miR-193a-3p dictates the multi-chemoresistance of bladder cancer via repression of SRSF2/PLAU/HIC2 expression

Cell Death Dis

5638、H-bc

11

Nestin overexpression promotes the embryonic development of heart and brain through the regulation of cell proliferation.

Brain Res

NSC


MiRNA‐30a inhibits AECs‐II apoptosis by blocking mitochondrial fission dependent on Drp‐1

J Cell Mol Med.

A549


Intercellular Adhesion Molecule-1 Inhibits Osteogenic Differentiation of Mesenchymal Stem Cells and Impairs Bio-Scaffold-Mediated Bone Regeneration In Vivo

TISSUE ENGINEERING: Part A

MSC

9

Characterization of Cep85–a new antagonist of Nek2A that is involved in the regulation of centrosome disjunction

J Cell Sci.

U2OS


BRCC3 acts as a prognostic marker in nasopharyngeal carcinoma patients treated with radiotherapy and mediates radiation resistance in vitro

Radiation Oncology

HNE1、CNE2R

*编号为实验图对应号码

 




图1. Wu et al. Journal of Translational Medicine (2015) 13:105

图2. Yu et al. Neurochem Res (2015) 40:698–712

图3. Sun et al. Int J Mol Med (2014) 33: 1586-1596

 




图4. Lv et al. Cancer Letters 357 (2015) 105–113

图5. Qi et al. World J Gastroenterol (2015) 21(34): 9900-9915

图6. Sun et al.Int J Mol Sci(2014) 15:11126-11141

   




图7. Yang et al. J Environ Sci (2015) 30:81-89

图8. Mu et al. PLoS ONE (2015) 10(8): e0136607

图9. Chen et al. Journal of Cell Science (2015) 128, 3290-3303

   



图10. Deng et al. Molecular Cancer (2014) 13:234

图11. Liu et al. Brain Research (2015) 1-11