预优化DNA转染试剂-SHEP-克隆与表达-试剂-生物在线
预优化DNA转染试剂-SHEP

预优化DNA转染试剂-SHEP

商家询价

产品名称: 预优化DNA转染试剂-SHEP

英文名称: GenJet™ In Vitro DNA Tranfection Reagent for SHEP Cell

产品编号: SL100489-SHEP

产品价格: 0

产品产地: 美国

品牌商标: SignaGen

更新时间: null

使用范围: null

济南思科生物科技有限公司
  • 联系人 :
  • 地址 : 济南市经六路689号绿地·玫瑰坊A座734号
  • 邮编 : 250021
  • 所在区域 : 山东
  • 电话 : 159****9896
  • 传真 : 0531-85912162
  • 邮箱 : jnqsw@163.com; info@signagen-china.com

Description

GenJet™ DNA In Vitro Tranfection Reagent for SHEP is pre-optimized for transfecting SHEP cells. This human neuroblastoma cell line was cloned from a single primary tumor. Unlike other neuroblastoma cell, SHEP cells does not produce IGF-II, expresses five-fold fewer IGF-IRs than SH-SY5Y and dies in serum-free media or following exposure to metabolic stressors. 

Refer to the following optimal transfection conditions for maximal transfection efficiency on SHEP
 cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
Cell culture conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent

Incubation Time to Form GenJet™/DNA Complex
Presence of Serum/Antibiotics during Transfection
Change Medium After Transfection
Maximal Efficiency 

Transfection Results:
Reporter Gene
Plasmid

Efficiency (GFP %)


~85%
DMEM with 4.5 g/L glucose, 10% FBS

3:1
Serum-free DMEM with 4.5 g/L glucose

15 minutes at RT
Yes
Yes, ~5 Hours After Transfection
48 hours


EGFP
pEGFP-N3 (CMV promoter) 

80%

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer

Data Sheet.pdf