假尿苷酸合酶1(PUS1)重组蛋白

YB742Hu013
Recombinant Pseudouridylate Synthase 1 (PUS1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
 假尿苷酸合酶1(PUS1)重组蛋白[ PROPERTIES ]
Residues: Met1~Asp427
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: Q9Y606
Host: E. coli
Subcellular Location: Mitochondrion.
Purity: >90%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
 假尿苷酸合酶1(PUS1)重组蛋白0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 8.7
Predicted Molecular Mass: 51.2kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
 假尿苷酸合酶1(PUS1)重组蛋白which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
MG LQ L RA L LG AFG RW T L R LG PRP S CSP RMA GNAE PPPA GA ACPQ DR RS CS
GRA GG DRVWE DGE HPA KK LK SGG DEE RRE K PPK RKIV L LM AYS GKGY H GM
QRNVGSSQFK TIEDDLVSAL VRSGCIPENH GEDMRKMSFQ RCARTDKGVS AAGQVVSLKV
WLIDDILEKI NSHLPSHIRI LGLKRVTGGF NSKNRCDART YCYLLPTFAF AHKDRDVQDE
TYRLSAETLQ QVNRLLACYK GTHNFHNFTS QKGPQDPSAC RYILEMYCEE PFVREGLEFA
VIRVKGQSFM MHQIRKMVGL VVAIVKGYAP ESVLERSWGT EKVDVPKAPG LGLVLERVHF
EKYNQRFGND GLHEPLDWAQ EEGKVAAFKE EHIYPTIIGT ERDERSMAQW LSTLPIHNFS
ATALTAGGTG AKVPSPLEGS EGDGDTD