肌球蛋白重链8(MYH8)重组蛋白

YB421Ra011
Myosin Heavy Chain 8, Skeletal Skeletal Skeletal Skeletal Muscle, Muscle, Muscle, Muscle, Perinatal Perinatal Perinatal Perinatal (MYH8)
Organism: Organism: Organism: Organism: Rattus norvegicus norvegicus norvegicus norvegicus (Rat)
Instruction Instruction Instruction Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
7th Edition (Revised in May, 2013)
 肌球蛋白重链8(MYH8)重组蛋白[ PROPERTIES PROPERTIES PROPERTIES PROPERTIES ]
Residues: Residues: Residues: Residues: His40~Val240 (Accession # P04462),
with N-terminal His-Tag.
Host: E. coli
Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Cytoplasm, myofibril.
Purity: Purity: Purity: Purity: >95%
Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0EU per 1μg
 肌球蛋白重链8(MYH8)重组蛋白(determined by the LAL method).
Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 6.4
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 24.9kDa
Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
 肌球蛋白重链8(MYH8)重组蛋白Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-H TQNASLINAK KKLENDVSQL QSEVEEVIQR ARNAEEKAKK
AITDAAMMAE ELKKEQDTSA HLERMKKNME QTVKDLQHRL DEAEQLALKG GKKQIQKLEA
RVRELEGEVE NEQKRNAEAV KGLRKHERRV KELTYQTEED RKNVLRLQDL VDKLQAKVKS
YKRQAEEAEE QSNVNLAKFR KLQHELEEAE ERADIAESQV