Diaphanous同源物1(DIAPH1)重组蛋白

YB265Hu014(DIAPH1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
 Diaphanous同源物1(DIAPH1)重组蛋白 PROPERTIES ]
Residues: Asp389~Pro583 (Accession # O60610),
with two N-terminal Tags, His-tag and GST-tag. Host: E. coli
Subcellular Location: Cell membrane. Cell
projection, ruffle membrane. Cytoplasm, cytoskeleton.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
 Diaphanous同源物1(DIAPH1)重组蛋白Predicted isoelectric point: 5.7
Predicted Molecular Mass: 54.5kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its
sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID
GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV
 Diaphanous同源物1(DIAPH1)重组蛋白DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK
KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR
GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-DF NEVFQILLNT
VKDSKAEPHF LSILQHLLLV RNDYEARPQY YKLIEECISQ IVLHKNGADP DFKCRHLQIE
IEGLIDQMID KTKVEKSEAK AAELEKKLDS ELTARHELQV EMKKMESDFE QKLQDLQGEK
DALHSEKQQI ATEKQDLEAE VSQLTGEVAK LTKELEDAKK EMASLSAAAI TVPPSVPSRA
PVP