EASYspin Plus 多糖多酚/复杂植物RNA快速提取试剂盒-核酸纯化-试剂-生物在线
EASYspin Plus 多糖多酚/复杂植物RNA快速提取试剂盒

EASYspin Plus 多糖多酚/复杂植物RNA快速提取试剂盒

商家询价

产品名称: EASYspin Plus 多糖多酚/复杂植物RNA快速提取试剂盒

英文名称: EASYspin Plus 多糖多酚/复杂植物RNA快速提取试剂盒

产品编号: RN5301

产品价格: 0

产品产地: 国产

品牌商标: 艾德莱

更新时间: null

使用范围: null

杭州昊鑫生物科技股份有限公司
  • 联系人 :
  • 地址 : 江干区天城路176号白云大厦2幢1003
  • 邮编 :
  • 所在区域 : 浙江
  • 电话 : 159****9305
  • 传真 : 0571-86955191
  • 邮箱 : 351778830@qq.com

本公司独家推出EASYspin无苯酚、氯仿RNA快速提取技术基础上,又独家研发成功基因组DNA清除柱技术可以有效清除gDNA残留,得到的RNA一般不需要DNase消化,可用于反转录PCR、荧光定量PCR等实验。独特的裂解液迅速裂解细胞和灭活细胞RNA酶, 离心沉淀去除多糖多酚和次级代谢产物,然后裂解混合物用乙醇调节RNA结合吸附到基因组DNA清除柱,然后RNA被选择性洗脱滤过, 吸附在基因组DNA清除柱上的残留DNA无法洗脱连同柱子一起丢弃从而清除掉DNA。滤过的RNA用乙醇调节结合条件后,RNA在高离序盐状态下选择性吸附于离心柱内硅基质膜,再通过一系列快速的漂洗-离心的步骤,去蛋白液和漂洗液将细胞代谢物,蛋白等杂质去除, 最后低盐的RNase free H20将纯净RNA从硅基质膜上洗脱。

产品特点:

1.完全不使用苯酚,氯仿等试剂,也不需要乙醇沉淀等步骤。 
2.简捷,单个样品操作一般可在25分钟内完成,世界上最简单快速的试剂盒。 
3.独家研发成功基因组DNA清除柱技术可以有效清除gDNA残留,得到的RNA一般不需要DNase消化,可用于反转录PCR、荧光定量PCR等实验。 
4.世界第一适应性极其广泛,可以提取包括复杂中草药如石斛/丹参/雪莲/人参、复杂淀粉种子如水稻/小麦/玉米种子、复杂果实如葡萄/蓝莓/草莓/西瓜果实、复杂抗逆植物如冬青/松针/沙棘/胡杨、复杂花卉月季/玫瑰/梅/牡丹花、复杂多糖植物紫菜/仙人掌/芦荟水稻种子等数百种国内外试剂盒提取失败的样品,使用EASYspin系列植物RNA提取试剂盒累计发表文章超过100篇。详细样品列表请参考公司主页产品介绍或者找我们索取100多篇发表文章原文。 
5.多次柱漂洗确保高纯度,OD260/OD280典型的比值达1.9~2.2,无DNA残留,可用于荧光定量PCR、RT-PCR、芯片、二代测序、Northern-blot等各种实验。
使用EASYspin/EASYspin Plus系列植物RNA提取试剂盒已经有大量文章发表,请联系我们索取发表的文章和包括北京林业大学,北京农学院,中国农业大学,农林科学院果树研究所,林业所,中国农科院等大学和研究院所大量使用案例。

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35. 棉花:Analysis of sea-island cotton and upland cotton in response to Verticillium dahliae infection by RNA sequencing. Sun et al. BMC Genomics 2013, 14:852 /1471-2164/14/852.

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42. 毛泡桐:Dynamic expression of novel and conserved microRNAs and their targets in diploid and tetraploid of Paulownia tomentosa. Biochimie xxx (2014) 1e10.

43. 人参:Cloning and Sequence Analysis Squalene Epoxidase Gene in Panax gin-seng. Journal of Jilin Agricultural University 2014, 36(2): 149-152,17

44. 雪莲:Cloning and Sequence Analysis of rbcs Gene from Sasussured involucrdta Kar. et Kir. Chinese Agricultural Science Bulletin 2014, 30(15): 261-267

45. 柑橘3Secreted Expression of Cecropin B Gene Enhances Resistance to Xanthomonas axonopodis pv. citri in Transgenic Citrus sinensisTarocco Acta Horticulturae Sinica 2014, 41(3): 417428 http: // www. ahs. ac. cn

46. 菊花:Stem apex detoxification culture markedly improved severalphysiological characters of chrysanthemum ‘YUTAI’. Plant Cell Tiss Organ Cult 2014, DOI 10.1007/s11240-014-0541-1

47. 荞麦和拟南芥:Ectopic expression of FaesAP3, a Fagopyrum esculentum (Polygonaceae) AP3 orthologous gene rescues stamen development in an Arabidopsis ap3 mutant. Gene 2014, 550(2): 200–206

48. 油松:Differential expression of SLOW WALKER2 homologue in ovules of female sterile mutant and fertile clone of Pinus tabulaeformis. Russian Journal of Developmental Biology 2014, 45(2): 78-84

49. 玫瑰花:Precise spatio-temporal modulation of ACC synthase by MPK6 cascade mediates the response of rose flowers to rehydration. The Plant Journal 2014, 79(6): 941–950

50. 棉花和拟南芥:Functional characterization of GhAKT1, a novel Shaker-like K+ channel gene involved in K+ uptake from cotton (Gossypium hirsutum). Gene 2014, 545(1): 61–71

51. 棉花和拟南芥1Upland Cotton Gene GhFPF1 Confers Promotion of Flowering Time and Shade-Avoidance Responses in Arabidopsis thaliana. PLoS ONE 2014, 9(3): e91869. doi:10.1371/journal.pone.0091869

52. 白杨:Poplar GATA transcription factor PdGNC is capable of regulating chloroplast ultrastructure, photosynthesis, and vegetative growth in Arabidopsis under varying nitrogen levels. Plant Cell Tiss Organ Cult 2014, DOI 10.1007/s11240-014-0536-y

53. 毛果杨:Molecular characterization of the SPL gene family in Populus trichocarpa. BMC Plant Biology 2014, 14: 131

54. 葛根:Molecular cloning and characterization of an isoflavone 7-O-glucosyltransferase from Pueraria lobata. Plant Cell Reports 2014, 33(7), 1173–1185

55. 百合:Cloning and Expression Analysis of Actin GenelilyActinfrom Lily. Acta Horticulturae Sinica 2013, 40(7): 13181326

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57. 黄鹌菜:Transcriptome Sequencing and De Novo Analysis of Youngia japonica Using the Illumina Platform. PLoS ONE 2014, 9(3): e90636. doi:10.1371/journal.pone.0090636

58. 棉花1Gibberellin Overproduction Promotes Sucrose Synthase Expression and Secondary Cell Wall Deposition in Cotton Fibers. PLoS ONE 2014, 9(5): e96537. doi:10.1371/journal.pone.0096537

59. 苹果:Low Medium pH Value Enhances Anthocyanin Accumulation in Malus Crabapple Leaves. PLoS ONE 2014, 9(6): e97904. doi:10.1371/journal.pone.0097904