BtrI
产品名称： BtrI
英文名称： BtrI
产品编号： HZ600388
产品价格： 0
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： null
- 联系人 : 鲍丽雯
- 地址 : 上海市闵行区闵北路88弄1-30号第22幢AQ136室
- 邮编 : 200612
- 所在区域 : 上海
- 电话 : 139****0749
- 传真 : 021-60345367
- 邮箱 : www.shzbio.net
BtrI
产品描述
使用说明
高浓度may result in星的酶活性。to obtain 100% activity，BSA should be added to the反应混合到最后1×100μg／ml浓度的BSA。do not use for long培养。
技术规格
Unit One Unit of the definition酶水解is required to the amount of DNA 1μgλ1小时60°c in at总量在50μl反应。
网站cac↑gtc recognition
嗜热脂肪芽孢杆菌是u62来源
我们assayedλDNA
优化缓冲区或50毫米的Tris-HCl（pH 7.6°C（10 mM MgCl2）；100毫米；NaCl；BSA DTT + 1毫米。）
60°c优化研究
10毫米的存储条件的Tris-HCl（pH 7.5）；50毫米氯化钾0.1毫米；EDTA；7毫米2 -巯基乙醇；200μg／ml甘油50 %的BSA；and；商店在零下20摄氏度。
在ligations 3倍80% of DNA酶overdigestion with can be ligated碎片。of these 90%可以收到。
非特异性水解后没有检测到孵化nonspecific activity was withλDNA 1μg of of of 5 16 hours for at UA听说是八周60°c酶。
甲基化效应不灵敏的测试
热灭活的酶灭活°C is at by潜伏期为20分钟。
ajii campatible结束？bmgbi ^ ^，
使用说明
High enzyme concentration may result in star activity. To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 60°C in a total reaction volume of 50 μl. |
| Recognition sites | CAC↑GTC |
| Source | Bacillus stearothermophilus SE-U62 |
| Assayed on | Lambda DNA |
| Optimal buffer | O (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA |
| Optimal temperature | 60°C |
| Storage conditions | 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol; Store at -20°C. |
| Ligations | After 3-fold overdigestion with enzyme 80% of DNA fragments can be ligated. Of these 90% can be recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 60°C. |
| Methlation effect | Methylation sensitivity not tested |
| Thermal inactivation | enzyme is inactivated by incubation at 80°C for 20 mins. |
| Campatible Ends | AjiI?^, BmgBI^ |