Human ICOS / AILIM / CD278 CHO Cell Lysate (WB positive control)北京义翘神州科技有限公司

产品详细描述

    
    



    

        
Product Description：
        
HEK293 Cell Human ICOS / AILIM / CD278 transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).

    
    

        
Expression Host：
        
HEK293 Cells
    
    

        
Species：
        
Human
    
    

        
Sequence Information：
        
A DNA sequence encoding the human ICOS (NP_036224.1) (Met1-Phe141) was expressed with the Fc region of rabbit IgG at the C-terminus.
    

        
Molecule Mass：
        
The recombinant human ICOS consists of 345 amino acids and predicts a molecular mass of 38.9 kDa.
    

        
Preparation Method：
        
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
    

        
Lysis Buffer：
        
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
    

        
Recommend Usage：
        
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 
2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 
3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃.
    

        
Sample Buffer：
        
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
    
    

      
Stability & Storage：
      
Store at 4℃. After re-dissolution, aliquot  and store at -80℃.
    
    

        
Application：
        
Western blot (WB): Use at an assay dependent dilution. 
Other Applications: Not tested.