性能参数
产品名称: Human ICOS / AILIM / CD278 CHO Cell Lysate (WB positive control)
英文名称: Human ICOS / AILIM / CD278 CHO Cell Lysate (WB positive control)
产品货号: 10344-H31HL
产品规格: 300μg
试剂级别: 分子生物学级
产品产地: 北京
产品商标: Sino Biological
价 格: 1560元
产品资料: 实验方法技术资料
更新时间: 2018/12/18 13:54:00
浏览人数:2
供应商: 北京义翘神州科技有限公司
诚信指数:899点
了解更多:进入公司展台
使用范围:仅限科研使用,不能应用于临床
生物在线声明:以上所展示的信息由企业自行提供,内容的真实性、准确性和合法性由发布企业负责。生物在线对此不承担任何保证责任。
产品详细描述
Product Description: | HEK293 Cell Human ICOS / AILIM / CD278 transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). |
Expression Host: | HEK293 Cells |
Species: | Human |
Sequence Information: | A DNA sequence encoding the human ICOS (NP_036224.1) (Met1-Phe141) was expressed with the Fc region of rabbit IgG at the C-terminus. |
Molecule Mass: | The recombinant human ICOS consists of 345 amino acids and predicts a molecular mass of 38.9 kDa. |
Preparation Method: | Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis Buffer: | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. |
Recommend Usage: | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. |
Sample Buffer: | 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). |
Stability & Storage: | Store at 4℃. After re-dissolution, aliquot and store at -80℃. |
Application: | Western blot (WB): Use at an assay dependent dilution. Other Applications: Not tested. |
生物在线声明:以上所展示的信息由企业自行提供,内容的真实性、准确性和合法性由发布企业负责。生物在线对此不承担任何保证责任。
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