Human CD86 / B7-2 CHO Cell Lysate (WB positive control)北京义翘神州科技有限公司

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产品名称: Human CD86 / B7-2 CHO Cell Lysate (WB positive control)
英文名称: Human CD86 / B7-2 CHO Cell Lysate (WB positive control)
产品货号: 10699-H03H-BL
产品规格: 300μg
试剂级别: 分子生物学级
产品产地: 北京
产品商标: Sino Biological
价    格: 1560元
产品资料: 实验方法技术资料
更新时间: 2018/7/12 17:13:00
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使用范围:仅限科研使用,不能应用于临床
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北京义翘神州科技有限公司
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北京亦庄东区科创七街31号院
邮编:
100176
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400-890-9989
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010-51029969
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北京·北京
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产品详细描述




    
    


Product Description: HEK293 Human CD86 / B7-2 transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).
Expression Host: HEK293
Species: Human
Sequence Information: A DNA sequence encoding the human CD86 (NP_008820.2) (Met1-His239) was expressed with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus. The purified protein was biotinylated in vitro.
Molecule Mass: The recombinant human CD86 consists of 467 amino acids and predicts a molecular mass of 53.1 kDa.
Preparation Method: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis Buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Recommend Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃.
Sample Buffer: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage: Store at 4℃. After re-dissolution, aliquot and store at -80℃.
Application: Western blot (WB): Use at an assay dependent dilution. Other Applications: Not tested.

生物在线声明:以上所展示的信息由企业自行提供,内容的真实性、准确性和合法性由发布企业负责。生物在线对此不承担任何保证责任。

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