重组人HVEM蛋白（Recombinant Human HVEM Protein），GMP级

产品货号：GMP-TL722

产品描述（Description）

Herpesvirus entry mediator (HVEM) is also known as TNFRSF14, TR2 (TNF receptorlike molecule) and ATAR (another TRAF associated receptor), is a type I membrane protein belonging to the TNF/NGF receptor superfamily. HVEM expression has been detected in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. The extracellular domain of HVEM has been shown to interact directly with the herpes simplex virus envelope glycoprotein D (gD). Two TNF superfamily ligands, including the secreted TNFβ (lymphotoxin α) and the membrane protein LIGHT (lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes), have been shown to be the cellular ligands for HVEM. Besides HVEM, LIGHT can also interact with LTβR, the receptor for lymphotoxin αβ heterotrimer. The role of the HVEM LIGHT /LTβ receptor ligand pair in immune function and herpesvirus pathobiology remains to be elucidated.

分子别名（Synonym）

TNFRSF14、ATAR、HVEA、LIGHTR、TR2、CD270

表达宿主（Expression host）

HEK293细胞

来源（Source）

Human

蛋白序列（Protein Sequence）

DNA 序列编码人 HVEM（ UniProtKB/Swiss-Prot:Q01973）表达带有 Fc 标签在 C 末端

分子量（Molecular Weight）

重组人HVEM蛋白 包含468个氨基酸，预测理论分子量为51.3 kD

纯度（Purity）

≥95%采用 SDS-PAGE 凝胶和高效液相色谱分析

内毒素（Endotoxin）

＜0.1EU/ug

生物活性（Biological Activity）

固定 LIGHT(His 标签) at 5 μg/mL (100 μL/well) 能够结合人 HVEM(Fc 标签) 的线性范围是 50-200ng/mL

稳定性（Stability）

冻干的样本的可以在4℃保存24个月，溶解后的液体可以于-20℃保存6-12个月，并且避免反复冻融 

组成（Component）

含有6%甘露醇的无菌PBS（PH 7.4）的冻干粉

参考文献

1、A TNFRSF14-FcɛRI-mast cell pathway contributes to development of multiple features of asthma pathology in mice Riccardo Sibilano, Nicolas Gaudenzio, Marianne K. DeGorter, Laurent L. Reber, Joseph D. Hernandez, Philipp M. Starkl, Oliwia W. Zurek, Mindy Tsai, Sonja Zahner, Stephen B. Montgomery, Axel Roers, Mitchell Kronenberg, Mang Yu, Stephen J. Galli Nat Commun. 2016; 7: 13696. Published online 2016 Dec 16. doi: 10.1038/ncomms13696 

2.、TNFRSF14 aberrations in follicular lymphoma increase clinically significant allogeneic T-cell responses Eleni Kotsiou, Jessica Okosun, Caroline Besley, Sameena Iqbal, Janet Matthews, Jude Fitzgibbon, John G. Gribben, Jeffrey K. Davies Blood. 2016 Jul 7; 128(1): 72–81. Prepublished online 2016 Apr 21. doi: 10.1182/blood-2015-10-679191

  注意：For research and manufacturing use.