ChIP DNA Clean & Concentrator™-核酸纯化-试剂-生物在线
ChIP DNA Clean & Concentrator™

ChIP DNA Clean & Concentrator™

商家询价

产品名称: ChIP DNA Clean & Concentrator™

英文名称: ChIP DNA Clean & Concentrator™

产品编号: D5201

产品价格: 0

产品产地: 美国

品牌商标: ZYMO RESEARCH

更新时间: null

使用范围: null

北京天漠科技开发有限公司
  • 联系人 :
  • 地址 : 北京市朝阳区北苑路170号凯旋城E座1809室
  • 邮编 : 100101
  • 所在区域 : 北京
  • 电话 : +86-10-139****7531
  • 传真 : +86-10-58235289
  • 邮箱 : fjan@zymoresearch.com.cn

 
ChIP DNA Clean & Concentrator™
 
Column design allows for small (≥ 6 µl) elution volumes.
DNA is ideal for PCR, arrays, DNA quantification, Southern blot analysis, sequencing, and other molecular applications.
Can also be used with cell lysates, Proteinase K digested samples, PCRs and other enzymatic reactions.
 
DNA Purity: High quality, purified DNA is eluted with elution buffer or water and is especially well suited for PCR amplification, arrays, Southern blot analysis, DNA quantification, sequencing, and other molecular applications.
DNA Size Limits: 50 bp to ~ 23 kb
DNA Recovery: For DNA 50 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%.
Sample Sources: Wherever DNA isolation and purification is required during standard ChIP protocols. This includes samples that have undergone reverse cross-linking and Proteinase K or RNase A digestion following either 1) mechanical or nuclease-mediated DNA shearing or 2) elution from chromatin-antibody-bead complexes in TES, 0.1M NaHCO3 and 1% SDS, or other buffers containing up to 1% SDS. This kit can also be used for DNA purification from PCR, enzymatic digestion, kinase, phosphatase and other enzymatic reactions.
Detergent tolerance: ≤ 5% Triton X-100, ≤ 5% Tween-20, ≤ 5% Sarkosyl, ≤ 0.1% SDS
 

The Chromatin Immunoprecipitation (ChIP) DNA Clean & Concentrator™ provides a hassle-free method for the rapid purification and concentration of high quality DNA from any step in a “standard” ChIP protocol. This includes samples that have undergone reverse cross-linking, Proteinase K or RNase A digestion, mechanical or nuclease-mediated DNA shearing, and samples eluted from chromatin-antibody-bead complexes. Additionally, this product may also be used to purify DNA from PCR and other enzymatic reactions.

The specially formulated ChIP DNA Binding Buffer promotes DNA adsorption to the column in the presence of detergents, antibodies, and proteinases that are often used for ChIP. Simply add the ChIP DNA Binding Buffer to your sample and transfer the mixture to the supplied Zymo-Spin™ Column. There is no need for organic extraction or ethanol precipitation. Instead, Fast-Spin column technology yields ultra-pure DNA in just minutes. The DNA purified using the ChIP DNA Clean & Concentrator™ is ideal for PCR amplification, arrays, DNA quantification, as well as other molecular applications. This kit may be applied to any routine ChIP procedure to determine DNA concentration of samples that have undergone reverse cross-linking following DNA shearing. It can also be used for the removal of TES, 0.1M NaHCO3, and 1% SDS from DNA eluted from chromatin-antibody-bead complexes and can be used to purify DNA from buffers containing up to 1% SDS or 5% NP-40, Tween-20, Triton X-100 or Sarkosyl.

The ChIP DNA Clean & Concentrator™ recovers ultra-pure DNA from cell lysates that is proportional to the lysate volume and DNA fragment size range (see ptotocol ).

 
Overview of ChIP DNA Clean & Concentrator™ Procedure

ORDERING INFORMATION

Product                                     Catalog No.       Size       Price(RMB)

ChIP DNA Clean & Concentrator™       

(uncapped column)                           D5201          50 preps 

(capped column)                             D5205             50 preps