L-Alanyl-L-Glutamine Solution (200 mM) is commonly called “Stable Glutamine”. This is a ready-to-use stock solution used to supply L-glutamine, an essential amino acid that is a required additive to most cell culture media. L-glutamine facilitates the storage and transfer of nitrogen to the cells in culture. Glutamine is also one of the most readily available amino acids for use as an energy source when glucose levels are low and energy demands are high, especially for rapidly proliferating cell types or cells cultured under hypoxic conditions, where glucose metabolism is less efficient.
In order to combat the rapid breakdown of free L-glutamine and increase glutamine stability within cell culture media, L--alanyl-L-glutamine dipeptides is often substituted. L-alanyl-L-glutamine is a dipeptide that is stable in cell culture medium over longer periods of time, even at 37°C. The strong bonds within L-alanyl-L-glutamine stabilize the compound from degradation, while the L-glutamine itself is still readily accessible to the cells. Supplementing stem cell media with L-alanyl-L-glutamine can extend the shelf-life of the media at 4°C and greatly reduce the problems associated with the breakdown of glutamine into ammonia waste.
Ready-to-use 200 mM stock solution
Increases glutamine stability within cell culture media
Improves cell health by minimizing toxic ammonia build-up
Extends media shelf-life at 4°C
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