RNA纯化试剂盒
产品名称： RNA纯化试剂盒
英文名称： GENEzol TriRNA Pure Kit
产品编号： GZX100
产品价格： 0
产品产地： 台湾
品牌商标： Geneaid Biotech
更新时间： 2023-08-16T10:47
使用范围： null
- 联系人 :
- 地址 : 北京市海淀区马连洼北路九号东居兴业写字楼
- 邮编 : 100196
- 所在区域 : 北京
- 电话 : 158****0961
- 传真 : 010-62894435
- 邮箱 : 15810200961@163.com
GENEzol™ TriRNA Pure Kit (GZX050/GZX100/GZX200)
The GENEzol™ TriRNA Pure Kit is a phenol and guanidine isothiocyanate plus spin column system for convenient purification of high-quality total RNA from a variety of samples. Initially, samples are homogenized in GENEzol™ Reagent without chloroform phase separation or isopropanol RNA precipitation. Following sample homogenization, simply bind, wash and elute the high-quality, total RNA in RNase-free Water and use in a variety of sensitive downstream applications.
Advantages (Cat. # GZX050, GZX100, GZX200)
•Purify total RNA in 15 minutes!
•A cost effective phenol, guanidine isothiocyanate solution plus spin column system
•No chlorophorm phase separation
•No isopropanol RNA precipitation
•No phenol carryover
•Sample Volume: up to 200 μl of blood, buffy coat, serum, plasma, up to 5 x 106 cultured cells, 10-50 mg of tissue, 1 x 109 bacterial cells, 20-50 mg of plant tissue
•High quality RNA: A260/A280 >1.8, A260/A230 >1.8
•Binding Capacity: 50 µg RNA from ≥ 25 µl DNase/RNase-free Water
•Spin Columns: glass fiber membrane optimized for total RNA extraction (certified RNase and DNase-free)
•Storage: GENEzol™ Reagent should be stored at 2ºC to 25ºC, other components should be stored at room temperature (15-25ºC)
Applications
cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays, Northern Blotting
Components
•GENEzol™ Reagent
•Pre-Wash Buffer
•Wash Buffer
•RNase-free Water
•RB Columns
•2 ml Collection Tubes
Quality Control
The GENEzol™ TriRNA Pure Kit is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system.
GENEzol™ TriRNA Pure Kit Interactive Protocol
GENEzol™ TriRNA Pure Kit Functional Test Data
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Figure 1. RNA was purified using the GENEzol™ TriRNA Pure Kit in parallel to the similar product from competitor Z. 5x105 HeLa cells were homogenized using GENEzol™ Reagent and competitor Z tri reagent. RNA was then purified using the corresponing kits spin column procedure. 10 µl from a 50 µl eluate of purified RNA was analyzed by electrophoresis on a 0.8% agarose gel.
Table 1. Total RNA purified using the GENEzol™ TriRNA Pure Kit and competitor Z. |
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Table 2. RNA was purified using the GENEzol™ TriRNA Pure Kit. RNA from 200 µl of whole human blood (3 donors) was initally homogenized using GENEzol™ Reagent. RNA was then purified using a spin column procedure. 10 µl from each 35 µl eluate of purified RNA was analyzed by electrophoresis on a 0.8% agarose gel. |
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GENEzol™ TriRNA Pure Kit real-time PCR Data
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RNA was extracted from 200 µl of human blood (5.2 x 1010 copy/ml) using GENEzol™ TriRNA Pure Kit. |
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RNA standard (107 copy/ml) RNA standard (106 copy/ml) RNA standard (105 copy/ml) |
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RNA standard (104 copy/ml) |
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Figure 3. Quantitative analysis of human beta globin mRNA purified by GENEzol™ TriRNA Pure Kit using a Taqman probe 1-step qRT-PCR assay. The assay was run on a BioRad IQ5 thermal cycler.
北京智杰方远代理台湾旭基――RNA纯化试剂盒-GENEzol TriRNA Pure Kit，GZX100现货促销.
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