2019-nCoV 新型冠状病毒 Spike HEK293 细胞裂解液 (WB positive control)-蛋白质/抗原/多肽-试剂-生物在线
2019-nCoV 新型冠状病毒 Spike HEK293 细胞裂解液 (WB positive control)

2019-nCoV 新型冠状病毒 Spike HEK293 细胞裂解液 (WB positive control)

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产品名称: 2019-nCoV 新型冠状病毒 Spike HEK293 细胞裂解液 (WB positive control)

英文名称: SARS-CoV-2 (2019-nCoV) Spike HEK293 Cell Lysate (WB positive control)

产品编号: 40591-V02HL

产品价格: 0

产品产地: 北京

品牌商标: 义翘神州(SinoBiologi)

更新时间: null

使用范围: null

北京义翘神州科技有限公司
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  • 邮编 : 100176
  • 所在区域 : 北京
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  • 传真 : 010-50911666
  • 邮箱 : marketing@sinobiological.cn

SARS-CoV-2 (2019-nCoV) 新型冠状病毒 Spike HEK293细胞裂解液(WB)产品信息
产品描述:This 2019-nCoV CoV spike overexpression lysate was created in Human Cells and intented for use as a Western blot (WB) positive control. Purification of CoV spike protein (Cat: 40591-V02H) from the overexpression lysate was verified.
表达宿主:Human Cells
种属:2019-nCoV
蛋白构建信息:A DNA sequence encoding the 2019-nCoV spike protein S1 was expressed with the Fc region of human IgG1 at the C-terminus.
分子量:The recombinant 2019-nCoV spike protein S1 consists of 908 amino acids and predicts a molecular mass of 101.7 kDa.

SARS-CoV-2 (2019-nCoV) 新型冠状病毒 Spike HEK293细胞裂解液(WB)产品信息使用指南
制备方法:Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解缓冲液:Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
使用建议:1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃.
缓冲液:1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件:Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
应用:Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.