SARS-CoV-2 N Protein Polyclonal Antibody-常用生化试剂-试剂-生物在线
SARS-CoV-2 N Protein Polyclonal Antibody

SARS-CoV-2 N Protein Polyclonal Antibody

商家询价

产品名称: SARS-CoV-2 N Protein Polyclonal Antibody

英文名称: SARS-CoV-2 N Protein Polyclonal Antibody

产品编号: Y0325

产品价格: 0

产品产地: 中国

品牌商标: 雅吉生物

更新时间: null

使用范围: null

上海雅吉生物科技有限公司
  • 联系人 :
  • 地址 : 上海市闵行区元江路5500号第1幢5658室
  • 邮编 :
  • 所在区域 : 上海
  • 电话 : 158****3937
  • 传真 : 021-34661275
  • 邮箱 : yajikit@163.com

WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.

H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.

配套提供了Western一抗稀释液,可以用于Western检测或其它适当用途时的一抗稀释。

建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):

WB IP IF IHC ICC FC ChIP ELISA
1:500-1:2000 - - - - - - 1:1000-1:5000

抗体详细信息如下:

About this Antibody
Name SARS-CoV-2 N Protein Polyclonal Antibody
Category Rabbit Polyclonal Antibody (pAb); Primary antibody
Isotype IgG
Purification method Antigen affinity purified
Positive samples -
Cellular location -
Customer validation -
About the Immunogen
Immunogen Recombinant full length of SARS-CoV-2 Nucleocapsid Protein
Sequence -
Gene ID 43740575
Swiss Prot -
Synonyms N protein; Nucleocapsid phosphoprotein; Nucleocapsid protein; N structural protein
Category Anti-infection
Background SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. SARS-CoV-2 Nucleocapsid Protein is associated with nucleic acid. It is the most abundant protein for coronavirus. Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the strong immunogenicity of coronavirus Nucleocapsid, it is believed that SARS-CoV-2 Nucleocapsid Protein has potential value for the diagnosis of the virus.

包装清单:

产品编号 产品名称 包装
AF0325 SARS-CoV-2 N Protein Polyclonal Antibody 50µl
AZ050 Western一抗稀释液 50ml
说明书 1份

保存条件:

SARS-CoV-2 N Protein Polyclonal antibody -20℃保存,Western一抗稀释液-20℃或4℃保存,一年有效。Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。

注意事项:

如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。

回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。

提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果,请考虑使用上述检测专用的一抗稀释液。

本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。

为了您的安全和健康,请穿实验服并戴一次性手套操作
请根据抗体的实际用途选择相应的使用方法。
1.Western检测:
a.按照推荐的稀释比例用提供的Western一抗稀释液稀释抗体。
b.把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时,确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c.回收稀释的一抗,4℃保存以备下次继续使用。
d.按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以
2.免疫染色:
可以使用生产的稀释抗体,使用后注意回收稀释好的一抗,
3.其它实验操作请自行参考适当的protocol进行。
4.代表性图片:


Fig. 1. Western blot using SARS-CoV-2 N Protein Polyclonal Antibody shows detection of a 48 kDa band corresponding to the protein.

Fig. 2. ELISA analysis of with SARS-CoV-2 N Protein Polyclonal Antibody. Antigen (1μg/mL). The antigen was used as the coating antigen, and the SARS-CoV-2 N Protein Polyclonal Antibody was used as the capture antigen for ELISA.