Description

The vascular endothelial cells contribute to the maintenance of vascular homeostasis. They synthesize and secrete activators as well as inhibitors of both the coagulation system and the fibrinolysis system, and mediators that influence the adhesion and aggregation of blood platelets. Endothelial cells also release molecules that control cell proliferation and modulate vessel wall tone. Activation of adenosine receptors on human pulmonary artery endothelial cell by lipopolysacchrides induces the release of IL-6 which may contribute to the pathophysiology of acute lung injury [1]. Many of these, and other, processes can be studied in vitro using cultured cells and human pulmonary artery derived endothelial cells are a commonly used cell type for such studies.

HPAEC from ScienCell Research Laboratories are isolated from human pulmonary artery. HPAEC are cryopreserved at secondary culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HPAEC are characterized by immunofluorescent method with antibodies to VWF/Factor VIII and CD31 (P-CAM) and by uptake of DiI-Ac-LDL. HPAEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPAEC are guaranteed to further expand for 15 population doublings at the condition provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Endothelial Cell Medium (ECM, Cat. No. 1001) for the culturing of HPAEC.

Product Use

HPAEC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Shipping

Dry ice.

Reference

[1] Wilson, C. N., Batra, V. K. (2002) Lipopolysaccharide binds to and activates A(1) adenosine receptors on human pulmonary artery endothelial cells. J. Endotoxin Res. 8(4):263-71.