上海复蒙基因生物科技有限公司是美国Sciencell公司中国一级代理:
美国ScienCell研究实验室(www.sciencellonline.com)成立于1999年,公司总部位于美国加州的圣地亚哥。主要致力于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发,在全球拥有众多客户。在国内销售8年来,很多老师应用其产品发表了高质量的SCI文章,且有着极高的文献引用率。凭借着严格的质控和优秀的产品品质,深受广大科研工作者的信赖。
ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制,细胞纯度可达98%。其中包括21种人体正常细胞系统,90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离,产品质量过硬。确保了实验结果的重复性和连贯性。
请登录Sciencell公司官方网站(www.sciencell-sh.com或www.sciencellonline.com)以确保购买正规公司产品。
人肺动脉成纤维细胞说明:
货号:3120
成纤维细胞是来源于胚胎中胚层的间充质细胞。成纤维细胞被广泛地用于细胞和分子领域的研究。由于成纤维细胞易于体外培养,且耐受性好,从而使可用于从基因转染到显微注射等多种操作。有确凿的证据表明在身体的不同部位,成纤维细胞有着本质上的区别。不同的刺激作用于肺动脉血管细胞,均可导致诸如血管壁中层结缔组织增多等一系列具有显著特点的血管重塑。这一病变的致病机理之一就是血管成纤维细胞的功能紊乱。在组织低氧引起肺动脉高压的发生过程中所观察到的外膜增厚就是由这种反应造成的。
人肺动脉成纤维细胞(HPAF)提取于人肺动脉组织,原代冻存。每管含有细胞数>5×105 cells/ml,此细胞通过纺锤丝形态和Fibronectin免疫荧光染色验证,经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。细胞可以达到15倍增。
推荐培养基:(FM, Cat. No. 2301)
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Description
Fibroblasts are mesenchymal cells which derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different [1]. The pulmonary arterial vesculature responds to various insults with a characteristic pattern of remodeling that includes an increase in connective tissue in the medial layer of the vessel wall [2]. One of the pathogenetic mechanisms involved is the functional derangements of vascular fibroblasts. Their proliferative response contributes to the adventitial thickening observed during the development of hypoxia-induced pulmonary hypertension [3].
HPAF from ScienCell Research Laboratories are isolated from human pulmonary artery. HPAF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HPAF are characterized by spindle morphology and by immunofluorescent method with antibody to fibronectin. HPAF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPAF are guaranteed to further expand for 15 population doublings at the condition provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium (FM, Cat. No. 2301) for the culturing of HPAF in vitro.
Product Use
HPAF are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Shipping
Dry ice.
Reference
[1] Conrad, G. W., Hart, G. W., Chen, Y. (1977) Differences in vitro between fibroblast-like cells from cornea, heart, and skin of embryonic chicks. J. Cell Sci. 26:119-137.
[2] Pendurthi, U. R., Rao, L. V. M., Williams, J. T. and Idell, S. (1999) Regulation of tissue factor pathway inhibitor expression in smooth muscle cells. Blood 94:579-586.
[3] Das M, Dempsey EC, Reeves JT, Stenmark KR. (2002) Selective expansion of fibroblast subpopulations from pulmonary artery adventitia in response to hypoxia. Am J Physiol Lung Cell Mol Physiol 282(5):L976-86.
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7.肺部细胞系统
3000 人肺微血管内皮细胞 (HPMEC)( 5×105 )
3100 人肺动脉内皮细胞(HPAEC) ( 5×105 )
3110 人肺动脉平滑肌细胞(HPASMC) ( 5×105 )
3120 人肺动脉成纤维细胞 (HPAF)( 5×105 )
3200 人肺泡上皮细胞 (HPAEpiC)( 1×106 )
3210 人支气管上皮细胞(HBEpiC)(5×105 )
3220 人气管上皮细胞(HTEpiC)(5×105 )
3230 人小气管上皮细胞(HSAEpiC)(5×105 )
3300 人肺成纤维细胞 (HPF)( 5×105 )
3400 人支气管平滑肌细胞 (HBSMC)( 5×105 )
3410 人气管平滑肌细胞 (HTSMC)( 5×105 )
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复蒙基因|FMGBio(www.fmgbio.com/ www.sciencell-sh.com/ )成立于2005年, 是专业从事牛血清生产、销售高端的原代细胞类科研产品。这些产品从根本上提高了全球生命医学研究、人类重要疾病药物研发、新药研发的成功率。近几年的新药研发成功率在逐年下降,其根本原因之一就是传统的药物筛选系统是建立在只具有30-40%人类基因群的一系列细胞株上。这样一个有“缺陷型”药物筛选系统所产生的药物用于人体上就会出现许多致命的弱点和不完整性。新一代药物筛选系统是含有一系列近乎完整的人类基因群的原代细胞株。而利用这些原代细胞株所甄别和筛选出来的候选药物,其诊治人类疾病的成功几率将大大增加。这样不但大大节省了新药的开发成本,而且将极大地提高人类的健康质量。
复蒙基因|FMGBio现有的产品:美国Sciencell(原代细胞、原代细胞专用培养基、无血清培养基)、3D Bioteck(三维细胞培养支架)、年产1000万毫升的内蒙古牛血清生产基地等。
复蒙基因|FMGBio的PI团队已经发展到专职PI 20人,联席PI 312人,其中大多数拥有海外背景。截止到2009年3月,以PI或联席PI为第一作者发表SCI 论文1682 篇(总IF 值为5721.82,其中影响因子IF>5.0 的论文216 篇,IF>10.0论文32 篇)。
电话:021-51262076 13371892488 13816636051
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网址:www.fmgbio.com/ www.sciencell-sh.com/
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