Description

Trabecular meshwork cells (TMC) play an active role in the aqueous outflow mechanism. Specific receptors for neurotransmitters and neuropeptides, including epinephrine, acetylcholine, and neuropeptide Y, have been identified in TMC. In addition, a long list of vasoactive peptides and growth factors trigger intracellular signaling mechanisms in TM cells [1]. TMC synthesize a great variety of extracellular matrix proteins as well as metelloproteinases. TMC culture has provided an invaluable tool to characterize pharmacological properties of TMC [2].

TMC from ScienCell Research Laboratories are isolated from juxtacanalicular and corneoscleral region of the human eye. TMC are cryopreserved on primary culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HTMC are characterized by immunofluorescent method with antibodies to alpha-smooth muscle actin and fibronectin. TMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. TMC are guaranteed to further expand for 15 population doublings in the conditions specified by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. No. 2301) for the culturing of TMC in vitro.

Product Use

TMC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Shipping

Dry ice.

Reference

[1]. Artur Llobet, Xavier Gasull and Arcadi Gual (2003) Understanding trabecular meshwork physiology: A key to the control of intraocular pressure News Physiol Sci 18:205-209.
[2] Jun Ueda, Kelly K. Wentz-Hunter, E. Lillian Cheng, Takeo Fukuchi, Haruki Abe, and Beatrice Y.J.T. Yue (2000) Ultrastructural Localization of Myocilin in Human Trabecular Meshwork Cells and Tissues. J Histochem Cytochem 48:1321-1329.