重组HRV 3C Protease蛋白
产品名称: 重组HRV 3C Protease蛋白
英文名称: Recombinant HRV 3C Protease
产品编号: S3CP01
产品价格: 0
产品产地: 北京
品牌商标: 义翘神州(SinoBiological)
更新时间: null
使用范围: null
- 联系人 :
- 地址 : 北京亦庄东区科创十街18号院9号楼306室
- 邮编 : 100176
- 所在区域 : 北京
- 电话 :
- 传真 : 010-50911666
- 邮箱 : marketing@sinobiological.cn
官方网址:http://www.sinobiological.cn/Protease-Tools-HRV-3C-Protease-g-1646.html
Content
2000 U Recombinant HRV 3C Protease ( in 50 mM Tris, 150 mM NaCl, 1 mM EDTA, 50% Glycerol, pH 8.0 ) 100 μg Cleavage Control Protein ( lyophilized from sterile PBS , pH 7.4 ) 5 ml 10X HRV 3C Cleavage Buffer ( 1.5 M NaCl , 0.5 M Tris-HCl , pH 7.5 ) Description
HRV 3C Protease encoded by human rhinovirus 14 is a highly purified recombinant cysteine protease with a His-tag. Recombinant HRV 3C Protease is a ~20KDa single-chain protein containing approximately 189 amino acids with calculated pI 8.46. HRV 3C protease folds into two anti-parallel six-stranded β-barrels and the site cleft is located at the junction of the two β-barrels domains. The enzyme requires neither metal nor cofactors for activity. It has been demonstrated that the enzyme exhibits highest activity around neutral pH at temperature ranging from 22 to 37℃, even retaining robust activity at 4℃. Thus, cleavage can be performed at low temperature to enhance the stability of the target protein. The catalytic activity is insensitive to organic solvents (up to 10%); however, it can be strongly stimulated by high concentration of anions such as sulfate.
Specificity: The enzyme recognizes the cleavage site: Leu-Glu-Val-Leu-Phe-Gln-↓-Gly-Pro SDS-PAGE:
Molecular Weight: ~22KDa on SDS-PAGE 储存方法: Store HRV 3C Protease at –20°C. Store HRV 3C Cleavage Control Protein and Protease Cleavage Buffer at –20°C or 4°C. 纯度: 98% by SDS-PAGE. Quality Control: The purity of each lot is determined by SDS-PAGE. And the activity is ensured by cleavage test with a recombinant fusion protein for each lot. The solution of HRV 3C protease is filtered through 0.22μm sterile filter before package. Application
The high specificity of HRV 3C protease makes it an ideal tool for cleaving fusion proteins at definite cleavage sites. The fusion protein can be purified and cleaved by HRV 3C to obtain the target protein. The recombinant HRV 3C protease is easily removed by IMAC Ni-charged resin.
Activity definition
One unit of HRV 3C Protrease is defined as the amount of enzyme that will cleave >95% of 100μg HRV 3C cleavage control protein in 150mM NaCl, 50mM Tris-HCl pH 7.5, at 4℃ for 16h.
User Protocol
Starting conditions
Temperature: 4℃ Incubation time: 16 hours or overnight TUNEology 波长可调检测卡盒-->