人脑血管外膜成纤维细胞说明：

货号：1110
     

Description
 

Fibroblasts are mesenchymal cells which derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different [1]. The vascular adventitia is defined as the outermost connective tissue of vessels. One of the pathogenetic mechanisms involved is the functional derangements of vascular fibroblasts. Their proliferative response contributes to the adventitial thickening observed during the development of hypoxia-induced pulmonary hypertension [2].

HBVAF from ScienCell Research Laboratories are isolated from human brain vascular tissue. HBVAF are cryopreserved at first passage of primary culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HBVAF are characterized by spindle morphology and by immunofluorescent method with antibody to fibronectin. HBVAF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HBVAF are guaranteed to further expand for 15 population doublings at the condition provided by ScienCell Research Laboratories.
Recommended Medium
 

It is recommended to use Fibroblast Medium (FM, Cat. No. 2301) for the culturing of HBVAF in vitro.
Product Use
 

HBVAF are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
 

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Shipping
 

Dry ice.
Reference
 

[1] Conrad, G. W., Hart, G. W., Chen, Y. (1977) Differences in vitro between fibroblast-like cells from cornea, heart, and skin of embryonic chicks. J. Cell Sci. 26:119-137.
[2] Das M, Dempsey EC, Reeves JT, Stenmark KR. (2002) Selective expansion of fibroblast subpopulations from pulmonary artery adventitia in response to hypoxia. Am J Physiol Lung Cell Mol Physiol 282(5):L976-86

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