Mesenchymal stem cells (MSC) are a well-characterized population of adult stem cells. They have the potential to develop into mature cells that produce fat, cartilage, bone, tendons, and muscle [1, 2]. These properties in combination with their developmental plasticity have generated tremendous interest in the potential use of mesenchymal stem cells to replace damaged tissues. MSC cultured without serum in the presence of transformation growth factor will differentiate into chondrocytes, whereas MSC cultured in serum with ascorbic acid and *** will differentiate into osteoblasts. MSC has the capability for renewal and differentiation into various lineages of mesenchymal tissues. In essence MSC could be cultured to expand their numbers then transplanted to the injured site or after seeding in/on shaped biomimetic scaffold to generate appropriate tissue constructs.
It is recommended to use Mesenchymal Stem Cell Medium (MSCM, Cat. No. 7501) for the culturing of MMSC-bm in vitro.
MMSC-bm are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.
1. Kassem, M. Mesenchymal stem cells: biological characteristics and potential clinical applications. 2004. Cloning Stem Cells. 6(4):369-74.
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