MGP 
(Plasmid #26526)

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• PURPOSE

  (Empty Backbone)
• DEPOSITING LAB

  David Baltimore
• PUBLICATION

  OConnell et al Proc Natl Acad Sci U S A. 2009 Apr 28. 106(17):7113-8.
• PRICE

  $65 (USD)

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Available to Academic and Nonprofits Only

BACKBONE

• Vector backbone
  pMSCV puro
  (Search Vector Database)
• Backbone manufacturer
  Clontech
• Backbone size (bp)6300
• Vector type
  Retroviral
• Selectable markers
  Puromycin

GROWTH IN BACTERIA

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  DH5alpha
• Copy number
  Unknown

SEQUENCE INFORMATION

• Depositor Sequences
  None.
• Addgene Sequences
  Partial (1)

Full plasmid sequence is available only if provided by the depositing laboratory.

GENE/INSERT

• Gene/Insert name
  None
• Tag / Fusion Protein
  • GFP (N terminal on backbone)

CLONING INFORMATION

• Cloning methodRestriction Enzyme
• 5′ cloning siteBglII (not destroyed)
• 3′ cloning siteEcoRI (not destroyed)
• 5′ sequencing primerpLXSN-5
• 3′ sequencing primerMSCV-rev
(Common Sequencing Primers)

RESOURCE INFORMATION

• Terms and Licenses
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials

DEPOSITOR COMMENTS

MGP is a modified pMSCV vector (Clontech) where GFP was placed downstream of the 5′ LTR.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

• For your Materials & Methods section:

  MGP was a gift from David Baltimore (Addgene plasmid # 26526)

• For your References section:

  Inositol phosphatase SHIP1 is a primary target of miR-155. OConnell RM, Chaudhuri AA, Rao DS, Baltimore D. Proc Natl Acad Sci U S A. 2009 Apr 28. 106(17):7113-8. 10.1073/pnas.0902636106 PubMed 19359473