分子量:41.2 kDa

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human Carbonic Anhydrase IX (38-414), His Tag by the LAL method.

Buffer:20 mM MES and 100 mM NaCl, pH6.5

生物活性:Measured by its esterase activity. The specific activity is measured with 1 mM 4-Nitrophenyl acetate and 2.5 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5 The specific activity is  >  15 pmoles / min / μg.

产品特性:Human Carbonic Anhydrase IX (38-414), His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 41.2 kDa. The predicted N-terminus is Gln 38. The reducing (R) protein migrates as 53-54 kDa in SDS-PAGE  due to glycosylation.

产品背景:Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes. CAs form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs rather slowly in the absence of a catalyst. One of the functions of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. The active site of most carbonic anhydrases contains a zinc ion. There are at least five distinct CA families (α, β, γ, δ and ε). 
Carbonic anhydrase 9 (CA9 / CAIX) is also known as Membrane antigen MN (MN), Renal cell carcinoma-associated antigen G250, which belongs to the alpha-carbonic anhydrase family. CA9 / CAIX with an optimal activity at pH 6.49. Reversible hydration of carbon dioxide. CA IX participates in pH regulation. CA9 may be involved in the control of cell proliferation and transformation. CA-IX appears to be a novel specific biomarker for a cervical neoplasia.