pET-30a质粒载体
产品名称: pET-30a质粒载体
英文名称: pET-30a质粒载体
产品编号: Biovector512363
产品价格: 0
产品产地: Biovector NTCC Inc. USA
品牌商标: Biovector, Addgene, ATCC, Invi
更新时间: null
使用范围: null
Biovector Co.,LTD
- 联系人 :
- 地址 : 北京市西直门外大街19号BioVector NTCC典型培养物保藏中心
- 邮编 : 100080
- 所在区域 : 北京
- 电话 : 189****8599
- 传真 : 010-53513060
- 邮箱 : Biovector@163.com
Product Data Sheet
Order ID |
Name |
Description |
Biovector-pET30a |
pET-30a(+) |
pET-30a(+),in E.coli,300uL,KanR.Storage:4℃ |
Recovery
- Obtain an LB agar plate with the appropriate antibiotic.
- Using a sterile pipette tip, touch the bacteria growing within the punctured area of the stab culture. (A sterilized wire loop or sterile toothpick can be used in place of a sterile pipette tip.)
- Run this tip lightly over a section of the plate, as shown in the figure, to create streak #1.
- Using another sterile pipette tip, pass through streak #1 and spread the bacteria over a second section of the plate, to create streak #2.
- Using a third sterile pipette tip, pass through streak #2 and spread the bacteria over the last section of the plate, to create streak #3.
- Grow overnight in a 37 o C incubator (unless a different growth temperature is indicated on the plasmid datasheet).