Product Data Sheet

Recovery

1. Obtain an LB agar plate with the appropriate antibiotic.
2. Using a sterile pipette tip, touch the bacteria growing within the punctured area of the stab culture. (A sterilized wire loop or sterile toothpick can be used in place of a sterile pipette tip.)
3. Run this tip lightly over a section of the plate, as shown in the figure, to create streak #1.
4. Using another sterile pipette tip, pass through streak #1 and spread the bacteria over a second section of the plate, to create streak #2.
5. Using a third sterile pipette tip, pass through streak #2 and spread the bacteria over the last section of the plate, to create streak #3.
6. Grow overnight in a 37 ^o C incubator (unless a different growth temperature is indicated on the plasmid datasheet).
7. In the morning, single colonies should be visible. If the bacterial growth is too dense, re-streak onto a new agar plate to obtain single colonies.

BACKBONE

• Vector backbone  pGL4.10 (luc2)
• Backbone manufacturer  Promega
• Backbone size w/o insert (bp)4200
• Vector type  Mammalian Expression, Luciferase

GROWTH IN BACTERIA

• Bacterial Resistance(s)  Ampicillin
• Growth Temperature  37°C
• Growth Strain(s)  DH5alpha
• Copy number  High Copy

GENE/INSERT