人雪旺细胞-细胞株/菌种-试剂-生物在线
人雪旺细胞

人雪旺细胞

商家询价

产品名称: 人雪旺细胞

英文名称: Human Schwann Cells

产品编号: XY1700

产品价格: 0

产品产地: 中国/美国

品牌商标: XYbscience

更新时间: 2023-08-17T09:55:27

使用范围: null

上海信裕生物科技有限公司
  • 联系人 : 徐经理
  • 地址 : 上海市闵行莘庄工业区春东路508号A1-2F
  • 邮编 : 200612
  • 所在区域 : 上海
  • 电话 : 152****8802
  • 传真 : 021-37680378
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人雪旺细胞Cell Specification
Schwann cells are neural crest derivatives that ensheathe and myelinate axons of peripheral
nerves [1]. They wrap individually around the shaft of peripheral axons, forming myelin sheath
along segments of the axon. Schwann cells play important roles in the development, function and
regeneration of peripheral nerves. When an axon is dying, the Schwann cells surrounding it aid
in its digestion. This leaves an empty channel formed by successive Schwann cells, through
which a new axon may grow from a severed end. The number of Schwann cells in peripheral
nerve is tightly regulated [2]. Their proliferation in vitro can be stimulated by growth factors
including PDGF, FGF, neuregulin and others [3]. The Schwann cells provide a relatively simple,
well-defined and accessible mammalian model for the study of a number of developmental
questions. It is also of particular clinical importance to understand the biology of Schwann cells,
not only in the context of neuropathies and nerve regeneration, but also because the cells or their
precursor might be especially well suited as implants to facilitate repair in the CNS.
HSC from ScienCell Research Laboratories are isolated from human spinal nerves. HSC are
cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml
volume. HSC are characterized by immunofluorescent method with antibodies to S-100, GFAP
and CD90. HSC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.
人雪旺细胞HSC are guaranteed to further culture in the conditions provided by ScienCell Research
Laboratories.
Recommended Medium
It is recommended to use Schwann Cell Medium (SCM, Cat. No. 1701) for the culturing of HSC
in vitro.
Product Use
HSC are for research use only. They are not approved for human or animal use, or for
application in in vitro diagnostic procedures.
Storage
Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep
the cells in liquid nitrogen until cell culture is needed for experiments.
Shipping
Dry ice.
Reference
[1] Jessen, K. R. and Mirsky, R. (1999) Schwann cells and their precursors emerge as major regulators of nerve
development. Trends Neurosci. 22:402-410.
[2] Syroid, D. E., Maycox, P. R., Burrola, P. G., Liu, N., Wen, D., Lee, K. F., Lemke, G., Kilpatrick, T. J. (1996)
Cell death in the Schwann cell lineage and its regulation by neuregulin. Proc. Natl. Acad. Sci. USA 93:9229-
9234.
[3] Rahmatullah, M., Schroering, A., Rothblum, K., Stahl, R. C., Urban, B and Carey, D. J. (1998) Synergistic
regulation of Schwann cells proliferation by heregulin and forskolin. Mol. Cell. Biol. 18:6245-6252.
Instruction for culturing cells
Caution: Cryopreserved cells are very delicate. Thaw the vial in a 37o
C waterbath
and return them to culture as quickly as possible with minimal handling!
Set up culture after receiving the order:
1. Prepare a poly-L-lysine coated flask (2 μg/cm2
, T-75 flask is recommended). Add 10 ml
of sterile water to a T-75 flask and then add 15 μl of poly-L-lysine stock solution (10
mg/ml, ScienCell cat. no. 0413). Leave the flask in incubator overnight (minimum one
hour at 37o
C incubator).
人雪旺细胞2. Prepare complete medium: decontaminate the external surfaces of medium and medium
supplements with 70% ethanol and transfer them to sterile field. Aseptically open each
supplement tube and add them to the basal medium with a pipette. Rinse each tube with
medium to recover the entire volume.
3. Rinse the poly-L-lysine coated flask with sterile water twice and add 20 ml of complete
medium to the flask. Leave the flask in the hood and go to thaw the cells.
4. Place the vial in a 37o
C waterbath, hold and rotate the vial gently until the contents are
completely thawed. Remove the vial from the waterbath immediately, wipe it dry, rinse
the vial with 70% ethanol and transfer it to a sterile field. Remove the cap, being careful
not to touch the interior threads with fingers. Using a 1 ml eppendorf pipette gently
resuspend the contents of the vial.
5. Dispense the contents of the vial into the equilibrated, poly-L-lysine coated culture
vessels. A seeding density of 10,000 cells/cm2
is recommended.
Note: Dilution and centrifugation of cells after thawing are not recommended since these
actions are more harmful to the cells than the effect of DMSO residue in the culture. It is
also important that Schwann cells are plated in poly-L-lysine coated culture vessels that
promote cell attachment.
6. Replace the cap or cover of flask, and gently rock the vessel to distribute the cells evenly.
Loosen cap if necessary to permit gas exchange.
7. Return the culture vessels to the incubator.
8. For best result, do not disturb the culture for at least 16 hours after the culture has been
initiated. Change the growth medium the next day to remove the residual DMSO and
unattached cells, then every other day thereafter. A healthy culture will display typical
oval-shaped cell body, with a prominent nucleus and bipolar extensions, giving an overall
spindle shape; and the cell number will be doubled after two to three days in culture.
Maintenance of Culture:
1. Change the medium to fresh supplemented medium the next morning after establishing a
culture from cryopreserved cells. For subsequent subcultures, change medium 48 hours
after establishing the subculture.
2. Change the medium every other day thereafter, until the culture is approximately 50%
confluent.
人雪旺细胞3. Once the culture reaches 50% confluence, change medium every day until the culture is
approximately 80% confluent.
Subculture:
1. Subculture the cells when they are 90% confluent.
2. Prepare poly-L-lysine coated cell culture flasks (2 μg/cm2
).
3. Warm medium, trypsin/EDTA solution, trypsin neutralization solution, and DPBS to
room temperature. We do not recommend warming the reagents and medium at 37o
C
waterbath prior to use.
4. Rinse the cells with DPBS.
5. Incubate cells with 10 ml of trypsin/EDTA solution (in the case of T-75 flask) until 80%
of cells are rounded up (monitored with microscope). Add 10 ml of trypsin neutralization
solution to the digestion immediately and gently rock the culture vessel.
Note: Use ScienCell Research Laboratories’ trypsin/EDTA solution that is optimized to
minimize the killing of the cells by over trypsinization.
6. Harvest and transfer released cells into a 50 ml centrifuge tube. Rinse the flask with
another 10 ml of growth medium to collect the residue cells. Examine the flask under
microscope to make sure the harvesting is successful by looking at the number of cells
left behind. There should be less than 5%.
7. Centrifuge the harvested cell suspension at 1000 rpm for 5 min and resuspend cells in
growth medium.
8. Count cells and plate them in a new, poly-L-lysine coated flask with cell density as
recommended.
Caution: Handling human derived products is potentially bioharzadous. Although each cell strain testes negative
人雪旺细胞for HIV, HBV and HCV DNA, diagnostic tests are not necessarily 100% accurate, therefore, proper precautions
mush be taken to avoid inadvertent exposure. Always wear gloves and safety glasses when working these materials.
Never mouth pipette. We recommend following the universal procedures for handling products of human origin as
the minimum precaution against contamination [1].
[1]. Grizzle, W. E., and Polt, S. S. (1988) Guidelines to avoid personal contamination by infective agents in research
laboratories that use human tissues. J Tissue Culture Methods. 11(4).