人少突胶质前体细胞--悬浮生长Cell Specification
Oligodendrocytes, the myelin-forming cells of the central nervous system, are postmitotic cells
that develop from oligodendrocyte precursor cells [1]. Oligodendrocytes, through their ability to
myelinate axons, play an essential role in the mature nervous system [2]. Although the function
of oligodendrocytes is well understood in rodent nervous system, little is known about the human
origin of this cell type. The study of human oligodendrocyte biology and the treatment of white
matter diseases would be facilitated by the ability to maintain cultures of enriched human
oligodendrocytes or their progenitors [3].
 人少突胶质前体细胞--悬浮生长HO from ScienCell Research Laboratories are derived from the differentiation of HOPC which
isolated from human brain tissue. HO are cryopreserved immediately after purification from
HOPC cultures and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. HO are
characterized by immunofluorescent method with antibodies to GalC and CNPase. HO are
negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HO are guaranteed to
further culture in the condition specified by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Oligodendrocyte Medium (OM, Cat. No. 1621) for human
oligodendrocyte culture in vitro.
Product Use
HO are for research use only. They are not approved for human or animal use, or for application
in in vitro diagnostic procedures.
Storage
Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep
the cells in liquid nitrogen until cell culture is needed for experiments.
Shipping
Dry ice.
Reference
 人少突胶质前体细胞--悬浮生长[1] Zhang, S.-C., Ge, B. and Duncan, I. D. (2000) Tracing human oligodendroglial development in vitro. J.
Neurosci. Res. 59:421-429.
[2] Woodruff, R. H. and Franklin, R. J. M. (1997) Growth factors and remyelination in the CNS. Histol. Histopathol.
12:459-466.
[3] Grever, W. E., Zhang, S.-C., Ge, B. and Duncan, I. D. (1999) Fractionation and enrichment of oligodendrocytes
from developing human brain. J. Neurosci. Res. 57:304-314.
Instruction for culturing cells
Caution: Cryopreserved cells are very delicate. Thaw the vial in a 37o
C waterbath
and return them to culture as quickly as possible with minimal handling!
Set up culture after receiving the ordering:
1. Place the vial in a 37o
C waterbath, hold and rotate the vial gently until the contents are
completely thawed. Remove the vial from the waterbath immediately, wipe it dry, and transfer
it to a sterile field. Rinse the vial with 70% ethanol, and then wipe to remove excess. Remove
the cap, being careful not to touch the interior threads with fingers.
2. Using 1 ml eppendorf pipette gently reused the contents of the vial and transfer the cells to a
15 ml conical centrifuge tube which contains 10 ml of complete OM (medium contains
Oligodendrocyte growth supplement). Centrifuge the tube at 1000 rpm for 5 min.
3. Discard the supernatant, gently reused the cells in complete OM, and dispense the cell
suspension into the equilibrated culture vessels (a T-25 flask). A high seeding density
(>10,000/cm2
) is recommended.
Note: It is important that neurons are plated in laminin, collagen, or poly-L-lysine coated culture
vessels that promote cell attachment and neurites outgrowth (Poly-L-lysine coating: coat flak
or plate with Poly-L-lysine for one hour and wash the flask or plate with sterile water three
times).
 人少突胶质前体细胞--悬浮生长4. Replace the cap or cover, and gently rock the vessel to distribute the cells evenly. Loosen
caps if necessary to permit gas exchange. Return the culture vessels to the incubator.
1. 5. Change medium every other day. A health culture will display normal oligodendrocyte
morphology, and nonvacuole cytoplasm with multiple processes.
Caution: Handling human derived products is potentially bioharzadous. Although each cell strain testes negative
for HIV, HBV and HCV DNA, proper precautions mush be taken to avoid inadvertent exposure. Always wear gloves
and safety glasses when working these materials. Never mouth pipette. We recommend following the universal
procedures for handling products of human origin as the minimum precaution against contamination [1].
[1]. Grizzle, W. E., and Polt, S. S. (1988) Guidelines to avoid personal contamination by infective agents in research
laboratories that use human tissues. J Tissue Culture Methods. 11(4).