人海马趾神经元Cell Specification
The tissue of the central nervous system is made up of two classes of cells that may be broadly
categorized as neurons and glia. Neurons are anatomic, functional, and trophic units of the brain
[1]. Despite great variability in size and shape, all neurons share common morphologic features,
which are those of the key elements of a highly complex communication network. The neurons
are the dynamically polarized cells that serve as the major signaling unit of the nervous system.
The human brain is known to contain about 1 x 1011 neurons, each being able to contact at least
10,000 other neurons [2]. The hippocampal neurons play a special role in learning and memory
and cultured hippocampal neurons are a useful model for studying neuronal phenomena such as
differentiation, survival, process growth, and synapto-genesis.
HHN from ScienCell Research Laboratories are isolated from hippocampal tissue of the brain.
HHN are cryopreserved at secondary cultures and delivered frozen. Each vial contains >1 x 106
cells in 1 ml volume. HHN are characterized by immunofluorescent method with antibodies to
neurafilament, MAP2, and beta-tubulin III. HHN are negative for HIV-1, HBV, HCV,
mycoplasma, bacteria, yeast and fungi. HHN are guaranteed to further culture in the conditions
provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use neuronal medium (NM, Cat. No. 1521) for the culture of human
人海马趾神经元neurons in vitro.
Product Use
HHN are for research use only. They are not approved for human or animal use, or for
application in in vitro diagnostic procedures.
Storage
Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep
the cells in liquid nitrogen until cell culture is needed for experiments.
Shipping
Dry ice.
Reference
[1] Parent, A. (1996) Neurons in Carpenters Human Neuroanatomy. 9th ed., pp131-198, Williams & Wilkins,
Quebec, Canada.
[2] Alberts, B., Bray, D., Lewis, J., Raff, M., Roberts, M., Watson, J. D. (1989) Molecular biology of the cell. 2nd.
ed., New York: Garland.
ScienCell
Research Laboratories
TM
Instruction for culturing cells
人海马趾神经元Caution: Cryopreserved cells are very delicate. Thaw the vial in a 37o
C waterbath
and return them to culture as quickly as possible with minimal handling!
Set up culture after receiving the ordering:
1. Place the vial in a 37o
C waterbath, hold and rotate the vial gently until the contents are
completely thawed. Remove the vial from the waterbath immediately, wipe it dry, and transfer
it to a sterile field. Rinse the vial with 70% ethanol, and then wipe to remove excess. Remove
the cap, being careful not to touch the interior threads with fingers.
2. Using 1 ml eppendorf pipette gently reused the contents of the vial and transfer the cells to a
15 ml conical centrifuge tube which contains 10 ml of complete NM (medium contains
neuronal growth supplement). Centrifuge the tube at 1000 rpm for 5 min.
3. Discard the supernatant, gently reused the cells in complete NM, and dispense the cell
suspension into the equilibrated culture vessels (a T-25 flask). A high seeding density
(>10,000/cm2
) is recommended.
Note: It is important that neurons are plated in laminin, collagen, or poly-L-lysine coated culture
vessels that promote cell attachment and neurites outgrowth (Poly-L-lysine coating: coat flask
or plate with Poly-L-lysine at 2 µg/ml concentration for one hour and wash the flask or plate
人海马趾神经元with sterile water three times).
4. Replace the cap or cover, and gently rock the vessel to distribute the cells evenly. Loosen
caps if necessary to permit gas exchange. Return the culture vessels to the incubator.
5. Change medium every other day. A health culture will display normal neuron morphology,
and nonvacuole cytoplasm with multiple processes.
Caution: Handling human derived products is potentially bioharzadous. Although each cell strain testes negative
for HIV, HBV and HCV DNA, proper precautions mush be taken to avoid inadvertent exposure. Always wear gloves
and safety glasses when working these materials. Never mouth pipette. We recommend following the universal
人海马趾神经元procedures for handling products of human origin as the minimum precaution against contamination [1].
[1]. Grizzle, W. E., and Polt, S. S. (1988) Guidelines to avoid personal contamination by infective agents in research
laboratories that use human tissues. J Tissue Culture Methods. 11(4).