人真皮成纤维细胞-胎儿丝裂霉素C处理-细胞株/菌种-试剂-生物在线
人真皮成纤维细胞-胎儿丝裂霉素C处理

人真皮成纤维细胞-胎儿丝裂霉素C处理

商家询价

产品名称: 人真皮成纤维细胞-胎儿丝裂霉素C处理

英文名称: Human Dermal Fibroblasts-Fetal-Mitomycin C treated

产品编号: XY2350

产品价格: 0

产品产地: 中国/美国

品牌商标: XYbscience

更新时间: 2023-08-17T09:55:27

使用范围: null

上海信裕生物科技有限公司
  • 联系人 : 徐经理
  • 地址 : 上海市闵行莘庄工业区春东路508号A1-2F
  • 邮编 : 200612
  • 所在区域 : 上海
  • 电话 : 152****8802
  • 传真 : 021-37680378
  • 邮箱 : shxysw02@163.com

人真皮成纤维细胞-胎儿丝裂霉素C处理Cell Specification
Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been
extensively used for a wide range of cellular and molecular studies as they are one of easiest
types of cells to grow in culture. Their durability also makes them amenable to a variety of
manipulations ranging from studies employing gene transfection to microinjection. In general,
fibroblasts secrete a non-rigid extracellular matrix which is rich in type I and/or type III collagen
[1]. There is evidence showing that fibroblasts in various organs are intrinsically different [2].
Dermal fibroblasts switch from a proliferative, migratory phase to a contractile, matrixremodeling
phase during wound healing. In addition, they secrete large quantities of hyaluronan
in response to inflammatory stimuli [3].
HDF-f-mt from ScienCell Research Laboratories are isolated from fetal human skin. HDF-f-mt
are cryopreserved at P0 and delivered frozen. Each vial contains > 5 x 105
cells in 1 ml volume.
人真皮成纤维细胞-胎儿丝裂霉素C处理HDF-f-mt are characterized by their spindle morphology and immunofluorescence with antibody
specific to fibronectin. HDF-f-mt are negative for HIV-1, HBV, HCV, mycoplasma, bacteria,
yeast, and fungi. HDF-f-mt are guaranteed to further culture under the conditions provided by
ScienCell Research Laboratories; however, HDF mt are not recommended for expanding as
mitomycin C treatment prevents further cell proliferation.
Recommended Medium
It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing HDF-f-mt in vitro.
Product Use
HDF-f-mt are for research use only. They are not approved for human or animal use, or for
application in in vitro diagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and
keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice.
References
[1] Gabbiani G, Rungger-Brandle E. (1981) “The fibroblast.” In Glynn LE, Handbook of Inflammation, Vol. 3:
Tissue Repair and Regeneration (pp 1-50). Amsterdam: Elsevier.
[2] Conrad GW, Hart GW, Chen Y. (1977) “Differences in vitro between fibroblast-like cells from cornea, heart,
and skin of embryonic chicks.” J Cell Sci. 26: 119-37.
[3] Stair S, Carlson KW, Shuster S, Wei ET, Stern R. (2002) “Mystixin peptides reduce hyaluronan deposition and
edema formation.” Eur J Pharmacol. 450: 291-6.
Instructions for culturing cells
Caution: Cryopreserved cells are very delicate. Thaw the vial in a 37oC water bath
and return the cells to culture as quickly as possible with minimal handling!
Initiating the culture:
1. Prepare 0.1% gelatin (Cat. #0423) coated culture vessel. Use enough volume of gelatin to
cover the entire culture surface. Leave the vessel in a 37oC incubator for 1 hour.
2. Prepare complete medium. Decontaminate the external surfaces of medium bottle and
medium supplement tubes with 70% ethanol and transfer them to a sterile field.
Aseptically transfer supplement to the basal medium with a pipette. Rinse the tube with
medium to recover the entire volume.
人真皮成纤维细胞-胎儿丝裂霉素C处理3. Completely aspirate gelatin from the coated vessel. It is not necessary to rinse the vessel.
Add complete medium to cover the culture surface. Leave the vessel in the sterile field
and proceed to thaw the cryopreserved cells.
4. Place the frozen vial in a 37oC water bath. Hold and rotate the vial gently until the
contents completely thaw. Promptly remove the vial from the water bath, wipe it down
with 70% ethanol, and transfer it to the sterile field.
5. Carefully remove the cap without touching the interior threads. Gently resuspend and
dispense the contents of the vial into a 15 ml conical centrifuge tube. Add appropriate
volume of complete medium to the tube, gently mix well and plate cells into the
equilibrated, gelatin-coated culture vessel at required plating density.
Note: Dilution and centrifugation of cells after thawing are not recommended since these
actions are more harmful to the cells than the effect of residual DMSO in the culture. It is
also important that cells are plated in gelatin coated culture vessels to promote cell
attachment.
6. Replace the cap or lid of the culture vessel and gently rock the vessel to distribute the
cells evenly. Loosen cap, if necessary, to allow gas exchange.
7. Return the culture vessel to the incubator.
8. For best results, do not disturb the culture for at least 16 hours after the culture has been
initiated. Refresh culture medium the next day to remove residual DMSO and unattached
cells. Cells are then ready for experiments as feeder layer.
HDF mt are not recommended to be subcultured as mitomycin C treatment prevents
further cell proliferation.
人真皮成纤维细胞-胎儿丝裂霉素C处理Caution: Handling human derived products is potentially biohazardous. Although each cell strain tests
negative for HIV, HBV and HCV DNA, diagnostic tests are not necessarily 100% accurate, therefore,
proper precautions must be taken to avoid inadvertent exposure. Always wear gloves and safety glasses
when working with these materials. Never mouth pipette. We recommend following the universal
procedures for handling products of human origin as the minimum precaution against contamination [1].
[1] Grizzle WE, Polt S. (1988) “Guidelines to avoid personal contamination by infective agents in research
laboratories that use human tissues.” J Tissue Cult Methods. 11: 191‐9.