人骨骼肌细胞-细胞株/菌种-试剂-生物在线
人骨骼肌细胞

人骨骼肌细胞

商家询价

产品名称: 人骨骼肌细胞

英文名称: Human Skeletal Muscle Cells

产品编号: XY3500

产品价格: 0

产品产地: 中国/美国

品牌商标: XYbscience

更新时间: 2023-08-17T09:55:27

使用范围: null

上海信裕生物科技有限公司
  • 联系人 : 徐经理
  • 地址 : 上海市闵行莘庄工业区春东路508号A1-2F
  • 邮编 : 200612
  • 所在区域 : 上海
  • 电话 : 152****8802
  • 传真 : 021-37680378
  • 邮箱 : shxysw02@163.com

人骨骼肌细胞Cell Specification
Skeletal muscle cells are one of the largest cells in the body. They are multinucleate formed by
the fusion of myoblasts. Skeletal muscle regeneration is a complex process in which many
factors are involved. When skeletal muscle suffers an injury, quiescent resident myoblasts called
satellite cells are activated to proliferate, migrate, and finally differentiate [1]. Various cellular
signaling pathways, such as phosphatidylinositol 3-kinase, calcineurin, Janus kinase 2/signal
transducer and activator of transcription 3 (STAT3), and mitogen-activated protein kinase
(MAPK) have been suggested to play an important role in skeletal muscle growth [2]. Insulinstimulated
glucose transport in cultured human skeletal muscle is mediated by GLUT4 and
heparan sulfate proteoglycan is involved in skeletal muscle differentiation [3]. The fusion of
mononucleated cells to form multinucleated myotubes is a central event in skeletal muscle
development. Controlling the onset and progression of this process is a complex set of
interactions between myoblasts and their environment. Skeletal muscle cell culture is a useful
model for studying this process of cell differentiation.
HSkMC from ScienCell Research Laboratories are isolated from human muscle of the pectoral
girdle. HSkMC are cryopreserved on primary culture and delivered frozen. Each vial contains >5
x 10^5 cells in 1 ml volume. HSkMC are characterized by immunofluorescent method with
antibodies to myosin, actin and actinin. HSkMC are negative for HIV-1, HBV, HCV,
mycoplasma, bacteria, yeast and fungi. HSkMC are guaranteed to further expand for 15
population doublings at the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Skeletal Muscle Cell Medium (SkMCM, Cat. No. 3501) for the
culturing of HSkMC in vitro.
人骨骼肌细胞Product Use
HSkMC are for research use only. It is not approved for human or animal use, or for application
in in vitro diagnostic procedures.
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep
the cells in liquid nitrogen until cell culture needed for experiments.
Shipping
Dry ice.
Reference
[1] Villena, J., Brandan, E. (2004) Dermatan sulfate exerts an enhanced growth factor response on skeletal muscle
satellite cell proliferation and migration. J Cell Physiol. 198(2):169-78.
[2] Morris, R. T., Spangenburg, E. E., Booth, F. W. (2004) Responsiveness of cell signaling pathways during the
failed 15-day regrowth of aged skeletal muscle. J Appl Physiol. 96(1):398-404.
[3] Al-Khalili, L., Chibalin, A. V., Kannisto, K., Zhang, B. B., Permert, J., Holman, G. D., Ehrenborg, E., Ding, V.
D., Zierath, J. R., Krook, A. (2004) Insulin action in cultured human skeletal muscle cells during differentiation:
assessment of cell surface GLUT4 and GLUT1 content. Cell Mol Life Sci. 60(5):991-8.
Instruction for culturing cells
Caution: Cryopreserved cells are very delicate. Thaw the vial in a 37o
C waterbath
and return them to culture as quickly as possible with minimal handling!
Set up culture after receiving the ordering:
1. Place the vial in a 37o
C waterbath, hold and rotate the vial gently until the contents are
completely thawed. Remove the vial from the waterbath immediately, wipe it dry, and
transfer it to a sterile field. Rinse the vial with 70% ethanol, and then wipe to remove
excess. Remove the cap, being careful not to touch the interior threads with fingers.
人骨骼肌细胞Using 1 ml eppendorf pipette gently resuspend the contents of the vial.
2. Dispense the contents of the vial into the equilibrated, poly-L-lysine coated culture
vessels. A seeding density of 7,500 cells/cm2
is recommended.
3. Note: Dilution and centrifugation of cells after thawing are not recommended since these
actions are more harmful to the cells than the effect of DMSO residue in the culture. It is
also important that fibroblasts are plated in poly-L-lysine coated culture vessels that
promote cell attachment.
4. Replace the cap or cover, and gently rock the vessel to distribute the cells evenly. Loosen
caps if necessary to permit gas exchange.
5. Return the culture vessels to the incubator.
6. For best result, do not disturb the culture for at least 16 hours after the culture has been
initiated. Change the growth medium the next day to remove the residual DMSO and
unattached cells, then every other day thereafter. A health culture will display fibroblastlike
morphology, nongranular cytoplasm, and the cell number will be doubled after two
to three days in culture.
Maintenance of Culture:
1. Change the medium to fresh supplemented medium the next morning after establishing a
culture from cryopreserved cells. For subsequent subcultures, change medium 48 hours
after establishing the subculture.
2. Change the medium every other day thereafter, until the culture is approximately 50%
confluent.
3. Once the culture reaches 50% confluence, change medium every day until the culture is
approximately 80% confluent.
Subculture:
1. Subculture the cells when they are 80% confluent.
2. Prepare poly-L-lysine coated cell culture flasks.
人骨骼肌细胞3. Warm medium, trypsin/EDTA solution, trypsin neutralization solution, and DPBS to
room temperature. We do not recommend warming the reagents and medium at 37o
C
waterbath prior to use.
4. Rinse the cells with DPBS.
5. Incubate cells with 3 ml of trypsin/EDTA solution (in the case of T-25 flask) until 80% of
cells are rounded up (monitored with microscope). Add 3 ml of trypsin neutralization
solution to the digestion immediately and gently rock the culture vessel.
6. Note: Use ScienCell Research Laboratories’ trypsin/EDTA solution that is optimized to
minimize the killing of the cells by over trypsinization.
7. Harvest and transfer released cells into a 15 ml centrifuge tube. Rinse the flask with
another 3 ml of growth medium to collect the residue cells. Examine the flask under
microscope to make sure the harvesting is successful by looking at the number of cells
left behind. There should be less than 5%.
8. Centrifuge the harvested cell suspension at 1000 rpm for 5 min and resuspend cells in
growth medium.
9. Count cells and plate them in a new, poly-L-lysine coated flask with cell density as
recommended.
Caution: Handling human derived products is potentially bioharzadous. Although each cell strain testes negative
for HIV, HBV and HCV DNA, diagnostic tests are not necessarily 100% accurate, therefore, proper precautions
mush be taken to avoid inadvertent exposure. Always wear gloves and safety glasses when working these materials.
人骨骼肌细胞Never mouth pipette. We recommend following the universal procedures for handling products of human origin as
the minimum precaution against contamination [1].
[1]. Grizzle, W. E., and Polt, S. S. (1988) Guidelines to avoid personal contamination by infective agents in research
laboratories that use human tissues. J Tissue Culture Methods. 11(4).